Rabbit Anti-ProProcaspase-3/PE抗体,荧光一抗说明书
Catalog Number : RS-06081R-PE
Quantity size : 100ug (2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10mg/ml BSA and 0.1% Sodium azide. )
Relevance: The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Reacts with Procaspase-3 subunit p17 and precursor.
Specificity : · anti- ProProcaspase-3/PE is a rabbit polyclonal antibody
· specific for anti-ProProcaspase-3/PE of hu,mo and rat
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt:17/28 kDa
ProProcaspase-3/PE抗体,荧光一抗说明书
Application : · Flow-cyt=1:50-200
.IF=1:50-200
· Excitation spectrum: 520-570nm
· Emission spectrum: 490-510nm
· not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
Storage: Store at –20 oC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 oC. ProProcaspase-3/PE抗体,荧光一抗说明书
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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