Fibrinogen抗体wb ihc操作

上海瑞齐生物技术有限公司

2014/05/29 08:29

Rabbit Anti-Fibrinogen抗体wb ihc操作 antibody

Catalog Number :RS-91240

Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ） Background: background:
Fibrinogen is the main protein of blood coagulation system. It is a large protein and it consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. All chains are connected with each other by a number of disulfide bonds. Fibrinopeptides A (1 to 16 amino acids) and B (1 to 17 amino acids) are released by thrombin from the N terminal parts of alpha and beta chains, respectively. In this way fibrinogen is converted into fibrin, which by means of polymerization forms a fibrin clot. Fibrinogen clotting underlies pathogenesis of MI, thromboembolism and thromboses of arteries and veins, since fibrin is the main substrate for thrombus formation. Fibrinogen activation is also involved in pathogenesis of inflammation, tumor growth and many other diseases. The normal fibrinogen concentration in plasma is about 3 mg/ml. The elevated level of fibrinogen in patient's blood is regarded as an independent risk factor for cardiovascular diseases. An increase in blood fibrinogen concentration was shown to be a strong predictor of coronary heart disease (Sonel A. et al, and Rapold H.J. et al). All these facts make fibrinogen an important parameter in the diagnosis of cardiovascular diseases.

Function:

Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

Specificity :

–Anti-Fibrinogen is a rabbit polyclonal antibody

–specific for Fibrinogen of rat ,mouse ,hu

– use for western blotting,elisa,immunoprecipitation and immunohistochemistry

–Protein G affinity chromatography purification, purity :>95%

– Isotype: IgG

– mol wt: 340 kDa

Application :

WB=1:100-500

ELISA=1:500-1000

IHC-P=1:100-500

IHC-F=1:100-500

IF=1:100-500Anti-Fibrinogen抗体wb ihc操作

– KLH conjugated synthetic peptide derived from human IL-8 C-terminus

– not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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