组蛋白去甲基化酶JARID2抗体

?组蛋白去甲基化酶JARID2抗体 

『别名』JARD2; JARD2_HUMAN; JARID2; JMJ; Jumonji AT rich interactive domain 2; Jumonji protein ; Jumonji/ARID domain containing protein 2; Jumonji/ARID domain-containing protein 2 ; Protein Jumonji. 

『浓度』组蛋白去甲基化酶JARID2抗体1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

组蛋白去甲基化酶JARID2抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

组蛋白去甲基化酶JARID2抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAD979Mu01 肾上腺素能受体β3(ADRβ3)多克隆抗体 Polyclonal Antibody to Adrenergic Receptor Beta 3 (ADRb3)

PAD979Mu02 肾上腺素能受体β3(ADRβ3)多克隆抗体 Polyclonal Antibody to Adrenergic Receptor Beta 3 (ADRb3)

PAD979Ra01 肾上腺素能受体β3(ADRβ3)多克隆抗体 Polyclonal Antibody to Adrenergic Receptor Beta 3 (ADRb3)

PAD978Mu01 肾上腺素能受体β2(ADRβ2)多克隆抗体 Polyclonal Antibody to Adrenergic Receptor Beta 2 (ADRb2)

PAD978Mu02 肾上腺素能受体β2(ADRβ2)多克隆抗体 Polyclonal Antibody to Adrenergic Receptor Beta 2 (ADRb2)

PAD978Ra01 肾上腺素能受体β2(ADRβ2)多克隆抗体 Polyclonal Antibody to Adrenergic Receptor Beta 2 (ADRb2)

PAC924Mu01 肾上腺素能受体β1(ADRβ1)多克隆抗体 Polyclonal Antibody to Adrenergic Receptor Beta 1 (ADRb1)

PAC924Mu02 肾上腺素能受体β1(ADRβ1)多克隆抗体 Polyclonal Antibody to Adrenergic Receptor Beta 1 (ADRb1)

PAC924Ra01 肾上腺素能受体β1(ADRβ1)多克隆抗体 Polyclonal Antibody to Adrenergic Receptor Beta 1 (ADRb1)

PAD977Hu01 肾上腺素能受体α2C(ADRα2C)多克隆抗体 Polyclonal Antibody to Adrenergic Receptor Alpha 2C (ADRa2C)

PAD977Ra01 肾上腺素能受体α2C(ADRα2C)多克隆抗体 Polyclonal Antibody to Adrenergic Receptor Alpha 2C (ADRa2C)

PAB298Hu01 肾上腺素能受体α1A(ADRα1A)多克隆抗体 Polyclonal Antibody to Adrenergic Receptor Alpha 1A (ADRa1A)

PAG315Mu01 ADP核糖转移酶1(ART1)多克隆抗体 Polyclonal Antibody to ADP Ribosyltransferase 1 (ART1)

PAG315Mu02 ADP核糖转移酶1(ART1)多克隆抗体 Polyclonal Antibody to ADP Ribosyltransferase 1 (ART1)

PAR517Hu01 ADP核糖基化因子样蛋白15(ARL15)多克隆抗体 Polyclonal Antibody to ADP Ribosylation Factor Like Protein 15 (ARL15)

PAB350Hu01 脂肪分化相关蛋白(ADRP)多克隆抗体 Polyclonal Antibody to Adipose Differentiation Related Protein (ADRP)

PAB350Hu02 脂肪分化相关蛋白(ADRP)多克隆抗体 Polyclonal Antibody to Adipose Differentiation Related Protein (ADRP)

PAA132Hu01 脂联素受体2(ADIPOR2)多克隆抗体 Polyclonal Antibody to Adiponectin Receptor 2 (ADIPOR2)

PAA132Ra01 脂联素受体2(ADIPOR2)多克隆抗体 Polyclonal Antibody to Adiponectin Receptor 2 (ADIPOR2)

PAA132Hu02 脂联素受体2(ADIPOR2)多克隆抗体 Polyclonal Antibody to Adiponectin Receptor 2 (ADIPOR2)

PAA147Bo01 脂联素受体1(ADIPOR1)多克隆抗体 Polyclonal Antibody to Adiponectin Receptor 1 (ADIPOR1)

PAA147Ga01 脂联素受体1(ADIPOR1)多克隆抗体 Polyclonal Antibody to Adiponectin Receptor 1 (ADIPOR1)

PAA147Hu01 脂联素受体1(ADIPOR1)多克隆抗体 Polyclonal Antibody to Adiponectin Receptor 1 (ADIPOR1)

PAA147Mu01 脂联素受体1(ADIPOR1)多克隆抗体 Polyclonal Antibody to Adiponectin Receptor 1 (ADIPOR1)

PAA147Po01 脂联素受体1(ADIPOR1)多克隆抗体 Polyclonal Antibody to Adiponectin Receptor 1 (ADIPOR1)

PAA147Ra01 脂联素受体1(ADIPOR1)多克隆抗体 Polyclonal Antibody to Adiponectin Receptor 1 (ADIPOR1)

PAA147Si01 脂联素受体1(ADIPOR1)多克隆抗体 Polyclonal Antibody to Adiponectin Receptor 1 (ADIPOR1)

PAA147Hu02 脂联素受体1(ADIPOR1)多克隆抗体 Polyclonal Antibody to Adiponectin Receptor 1 (ADIPOR1)

PAA147Mu02 脂联素受体1(ADIPOR1)多克隆抗体 Polyclonal Antibody to Adiponectin Receptor 1 (ADIPOR1)

PAA605Bo01 脂联素(ADP)多克隆抗体 Polyclonal Antibody to Adiponectin (ADP)

PAA605Ca01 脂联素(ADP)多克隆抗体 Polyclonal Antibody to Adiponectin (ADP)

PAA605Ga01 脂联素(ADP)多克隆抗体 Polyclonal Antibody to Adiponectin (ADP)

PAA605Hu01 脂联素(ADP)多克隆抗体 Polyclonal Antibody to Adiponectin (ADP)

PAA605Mu01 脂联素(ADP)多克隆抗体 Polyclonal Antibody to Adiponectin (ADP)

PAA605Po01 脂联素(ADP)多克隆抗体 Polyclonal Antibody to Adiponectin (ADP)

PAA605Ra01 脂联素(ADP)多克隆抗体 Polyclonal Antibody to Adiponectin (ADP)

PAA605Rb51 脂联素(ADP)多克隆抗体 Polyclonal Antibody to Adiponectin (ADP)

PAA605Mu02 脂联素(ADP)多克隆抗体 Polyclonal Antibody to Adiponectin (ADP)

PAQ372Hu01 脂肪细胞膜关联蛋白(APMAP)多克隆抗体 Polyclonal Antibody to Adipocyte Plasma Membrane Associated Protein (APMAP)

PAC282Hu01 粘附调节分子1(ADRM1)多克隆抗体 Polyclonal Antibody to Adhesion Regulating Molecule 1 (ADRM1)

PAQ889Hu01 Ig样域蛋白粘附分子1(AMIGO1)多克隆抗体 Polyclonal Antibody to Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1)

PAC830Hu01 腺苷酸环化酶关联蛋白2(CAP2)多克隆抗体 Polyclonal Antibody to Adenylyl Cyclase Associated Protein 2 (CAP2)

PAC830Hu02 腺苷酸环化酶关联蛋白2(CAP2)多克隆抗体 Polyclonal Antibody to Adenylyl Cyclase Associated Protein 2 (CAP2)

PAB349Hu01 腺苷酸环化酶关联蛋白1(CAP1)多克隆抗体 Polyclonal Antibody to Adenylyl Cyclase Associated Protein 1 (CAP1)

PAF205Hu01 腺苷酸激酶3(AK3)多克隆抗体 Polyclonal Antibody to Adenylate Kinase 3 (AK3)

PAB347Hu01 垂体腺苷酸环化酶激活肽(ADCYAP1)多克隆抗体 Polyclonal Antibody to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1)

PAB347Mu01 垂体腺苷酸环化酶激活肽(ADCYAP1)多克隆抗体 Polyclonal Antibody to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1)

PAB347Ra01 垂体腺苷酸环化酶激活肽(ADCYAP1)多克隆抗体 Polyclonal Antibody to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1)

PAB347Mu02 垂体腺苷酸环化酶激活肽(ADCYAP1)多克隆抗体 Polyclonal Antibody to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1)

PAB427Hu01 腺苷酸环化酶9(ADCY9)多克隆抗体 Polyclonal Antibody to Adenylate Cyclase 9 (ADCY9)

PAB427Mu01 腺苷酸环化酶9组蛋白去甲基化酶JARID2抗体(ADCY9)多克隆抗体 Polyclonal Antibody to Adenylate Cyclase 9 (ADCY9)

PAB420Hu01 腺苷酸环化酶8(ADCY8)多克隆抗体 Polyclonal Antibody to Adenylate Cyclase 8, Brain (ADCY8)

PAB419Hu01 腺苷酸环化酶7(ADCY7)多克隆抗体 Polyclonal Antibody to Adenylate Cyclase 7 (ADCY7)

PAB419Mu01 腺苷酸环化酶7(ADCY7)多克隆抗体 Polyclonal Antibody to Adenylate Cyclase 7 (ADCY7)