供货周期: | 一周 |
品牌: | Abcam |
规格: | 0.1ml/100μg 0.2ml/200μg |
货号: | Ab-07372 |
CAS号: | 此产品无CAS编号 |
磷酸化丝裂原活化蛋白激酶激酶2抗体
『别名』MEK2(Phospho-Thr394); Cardiofaciocutaneous syndrome; CFC syndrome; Dual specificity mitogen activated protein kinase kinase 2; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; FLJ26075; MAP kinase kinase 2; MAP2K 2; map2k2; MAPK / ERK kinase 2; MAPK/ERK kinase 2; MAPKK 2; MAPKK2; MEK 2; MEK2; Microtubule Associated Protein Kinase Kinase 2; Mitogen activated protein kinase kinase 2; Mitogen activated protein kinase kinase 2 p45; MKK 2; MKK2; MP2K2_HUMAN; OTTHUMP00000165826; OTTHUMP00000165827; PRKMK 2; PRKMK2 V.
『浓度』磷酸化丝裂原活化蛋白激酶激酶2抗体1mg/1ml
『规格』0.1ml/100μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
磷酸化丝裂原活化蛋白激酶激酶2抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
磷酸化丝裂原活化蛋白激酶激酶2抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
EL0757c豚鼠丙酮酸脱氢酶β(PDHβ)酶联免疫吸附测定试剂盒Guinea pig PDHβ (Pyruvate Dehydrogenase Beta) ELISA Kit
EL0118c豚鼠丙酮酸脱氢酶E1(PDH E1)酶联免疫吸附测定试剂盒Guinea pig PDH E1 (Pyruvate Dehydrogenase E1) ELISA Kit
EL0888c豚鼠血小板衍生生长因子受体β(PDGFRβ)酶联免疫吸附测定试剂盒Guinea pig PDGFRβ (Platelet Derived Growth Factor Receptor Beta) ELISA Kit
EL0501c豚鼠血小板衍生生长因子受体α(PDGFRα)酶联免疫吸附测定试剂盒Guinea pig PDGFRα (Platelet Derived Growth Factor Receptor Alpha) ELISA Kit
EL0595c豚鼠血小板衍生生长因子D(FDGFD)酶联免疫吸附测定试剂盒Guinea pig PDGFD (Platelet Derived Growth Factor D) ELISA Kit
EL0315c豚鼠血小板衍生生长因子BB(PDGF-BB)酶联免疫吸附测定试剂盒Guinea pig PDGF-BB (Platelet Derived Growth Factor-BB) ELISA Kit
EL0485c豚鼠血小板衍生生长因子AB(PDGF-AB)酶联免疫吸附测定试剂盒Guinea pig PDGF-AB (Platelet Derived Growth Factor AB) ELISA Kit
EL1209c豚鼠血小板衍生生长因子AA(PDGF-AA)酶联免疫吸附测定试剂盒Guinea pig PDGF-AA (Platelet Derived Growth Factor-AA) ELISA Kit
EL0028c豚鼠血小板衍生生长因子亚基A(PDGFA)酶联免疫吸附测定试剂盒Guinea pig PDGFA (Platelet Derived Growth Factor Subunit A ) ELISA Kit
EL0842c豚鼠血小板衍生生长因子(PDGF)酶联免疫吸附测定试剂盒Guinea pig PDGF (Platelet-Derived Growth Factor) ELISA Kit
EL0984c豚鼠磷酸二酯酶4D(PDE4D)酶联免疫吸附测定试剂盒Guinea pig PDE4D (Phosphodiesterase 4D, cAMP Specific) ELISA Kit
EL1239c豚鼠Pdgfa相关蛋白(PDAP1)酶联免疫吸附测定试剂盒Guinea pig PDAP1 (Pdgfa Associated Protein 1) ELISA Kit
EL1066c豚鼠降钙素原(PCT)酶联免疫吸附测定试剂盒 Guinea pig PCT (Procalcitonin) ELISA Kit
EL0918c豚鼠前蛋白转化酶枯草溶菌素9(PCSK9)酶联免疫吸附测定试剂盒 Guinea pig PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) ELISA Kit
EL1089c豚鼠前胶原C端蛋白酶增强子1(PCPE1)酶联免疫吸附测定试剂盒Guinea pig PCPE1 (Procollagen C Proteinase Enhancer 1 ) ELISA Kit
EL0716c豚鼠前胶原C端肽酶(PCP)酶联免疫吸附测定试剂盒 Guinea pig PCP (Procollagen C Peptidase)ELISA Kit
EL0467c豚鼠增殖细胞核抗原(PCNA)酶联免疫吸附测定试剂盒Guinea pig PCNA (Proliferating Cell Nuclear Antigen) ELISA Kit
EL0392c豚鼠磷酸烯醇式丙酮酸羧激酶1(PCK1)酶联免疫吸附测定试剂盒Guinea pig PCK1 (Phosphoenolpyruvate Carboxykinase 1) ELISA Kit
EL0841c豚鼠广谱细胞角蛋白(P-CK)酶联免疫吸附测定试剂盒Guinea pig P-CK (Pan-Cytokeratin) ELISA Kit
EL0854c豚鼠原钙黏素γA2(PCDHγA2)酶联免疫吸附测定试剂盒 Guinea pig PCDHγA2 (Protocadherin Gamma A2) ELISA Kit
EL0882c豚鼠原钙黏素15(PCDH15)酶联免疫吸附测定试剂盒 Guinea pig PCDH15 (Protocadherin 15) ELISA Kit
EL0583c豚鼠原钙黏素1(PCDH1)酶联免疫吸附测定试剂盒 Guinea pig PCDH1 (Protocadherin 1) ELISA Kit
EL1289c豚鼠正辅因子4(PC4)酶联免疫吸附测定试剂盒Guinea pig PC4 (Positive Cofactor 4) ELISA Kit
EL0174c豚鼠胆碱磷酸甘油酯(PC/CPG)酶联免疫吸附测定试剂盒 Guinea pig PC/CPG (Choline Phosphoglyceride) ELISA Kit
EL1165c豚鼠肺部活化调节趋化因子(PARC/CCL18)酶联免疫吸附测定试剂盒 Guinea pig PARC/CCL18 (Pulmonary Activation Regulated Chemokine) ELISA Kit
EL0504c豚鼠蛋白酶激活受体3(PAR3)酶联免疫吸附测定试剂盒Guinea pig PAR3 (Protease Activated Receptor 3) ELISA Kit
EL0214c豚鼠蛋白酶激活受体2(PAR2)酶联免疫吸附测定试剂盒Guinea pig PAR2 (Protease Activated Receptor 2) ELISA Kit
EL0814c豚鼠蛋白酶激活受体1(PAR1)酶联免疫吸附测定试剂盒Guinea pig PAR1 (Protease Activated Receptor 1) ELISA Kit
EL0533c豚鼠纤溶酶-抗纤溶酶复合物(PAP)酶联免疫吸附测定试剂盒Guinea pig PAP (Plasmin-Antiplasmin Complex) ELISA Kit
EL0917c豚鼠L苯丙氨酸解氨酶(PAL)酶联免疫吸附测定试剂盒 Guinea pig PAL (L-Phenylalanine Ammonla-Lyase) ELISA Kit
EL0309c豚鼠血小板相关免疫球蛋白(PAIg)酶联免疫吸附测定试剂盒 Guinea pig PAIg (Platelet Associated Immunoglobulins) ELISA Kit
EL0419c豚鼠纤溶酶原激活物抑制因子1(PAI1)酶联免疫吸附测定试剂盒 Guinea pig PAI1 (Plasminogen Activator Inhibitor 1) ELISA Kit
EL0083c豚鼠苯丙氨酸羟化酶 (PAH)酶联免疫吸附测定试剂盒Guinea pig PAH (Phenylalanine Hydroxylase) ELISA Kit
EL0192c豚鼠血小板激活因子ib3(PAFAHIB3)酶联免疫吸附测定试剂盒Guinea pig PAFAHIB3 (Platelet Activating Factor Acetylhydrolase Ib3) ELISA Kit
EL0523c豚鼠血小板激活因子(PAF)酶联免疫吸附测定试剂盒Guinea pig PAF (Platelet Activating Factor) ELISA Kit
EL0443c豚鼠垂体腺苷酸环化酶激活肽受体 (PACAPR)酶联免疫吸附测定试剂盒Guinea pig PACAPR (Pituitary Adenylate Cyclase Activating Polypeptide Receptor 1) ELISA Kit
EL1321c豚鼠垂体腺苷酸环化酶激活肽38(PACAP-38)酶联免疫吸附测定试剂盒 Guinea pig PACAP-38 (Pituitary Adenylate Cyclase Activating Polypeptide 38) ELISA Kit
EL1322c豚鼠垂体腺苷酸环化酶激活肽27(PACAP-27)酶联免疫吸附测定试剂盒Guinea pig PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit
EL1019c豚鼠血小板相关补体3(PAC3)酶联免疫吸附测定试剂盒 Guinea pig PAC3 (Platelet Associated Complement 3) ELISA Kit
EL0380c豚鼠Ⅲ型前胶原羧基端原肽(PⅢCP)酶联免疫吸附测定试剂盒Guinea pig PⅢCP (Procollagen Ⅲ C-Terminal ProPeptide) ELISA Kit
EL0910c豚鼠神经髓鞘蛋白(p2)酶联免疫吸附测定试剂盒 Guinea pig P2 (Myelin Protein 2) ELISA Kit
EL0058c豚鼠氧化低密度脂蛋白(OxLDL)酶联免疫吸附测定试剂盒Guinea pig OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit
EL1299c豚鼠卵清蛋白特异性IgG(OVA sIgG)酶联免疫吸附测定试剂盒 Guinea pig OVA sIgG (Ovalbumin Specific IgG) ELISA Kit
EL1112c豚鼠八聚体转录因子2A(OTF2A)酶联免疫吸附测定试剂盒 Guinea pig OTF2A (Octamer Transcription Factor 2A) ELISA Kit
EL0201c豚鼠抑瘤素M受体(OSMR)酶联免疫吸附测定试剂盒Guinea pig OSMR (Oncostatin M Receptor) ELISA Kit
EL0844c豚鼠抑瘤素M(OSM)酶联免疫吸附测定试剂盒Guinea pig OSM (Oncostatin M) ELISA Kit
EL1117c豚鼠破骨细胞刺激因子1 (OSF)酶联免疫吸附测定试剂盒Guinea pig OSF (Osteoclast Stimulating Factor 1) ELISA Kit
EL0134c豚鼠破骨细胞相关受体(OSCAR)酶联免疫吸附测定试剂盒Guinea pig OSCAR (Osteoclast Associated Receptor) ELISA Kit
EL0125c豚鼠类粘蛋白2(ORM2)酶联免疫吸附测定试剂盒 Guinea pig ORM2 (Orosomucoid 2) ELISA Kit
EL0171c豚鼠骨桥素(OPN)酶联免疫吸附测定试剂盒Guinea pig OPN (Osteopontin) ELISA Kit
EL0948c豚鼠骨保护素配体(OPGL)酶联免疫吸附测定试剂盒Guinea pig OPGL (Osteoprotegerin Ligand) ELISA Kit
EL1061c豚鼠骨保护素(OPG)酶联免疫吸附测定试剂盒 Guinea pig OPG (Osteoprotegerin) ELISA Kit
EL0732c豚鼠骨粘连蛋白(ON)酶联免疫吸附测定试剂盒Guinea pig ON (Osteonectin) ELISA Kit
EL0606c豚鼠抗氧化低密度脂蛋白抗体(OLAb)酶联免疫吸附测定试剂盒 Guinea pig OLAb (Anti-Oxidized Low Density Lipoprotein Antibody) ELISA Kit
EL0632c豚鼠癌蛋白诱导转录物3(OIT3)酶联免疫吸附测定试剂盒Guinea pig OIT3 (Oncoprotein Induced Transcript 3) ELISA Kit
EL0444c磷酸化丝裂原活化蛋白激酶激酶2抗体豚鼠成骨生长肽(OGP)酶联免疫吸附测定试剂盒 Guinea pig OGP (Osteogenic Growth Peptide) ELISA Kit
EL1203c豚鼠孤腓肽(OFQ/N)酶联免疫吸附测定试剂盒Guinea pig OFQ/N (Orphanin FQ/Nociceptin) ELISA Kit
EL1020c豚鼠鸟氨酸脱羧酶(ODC)酶联免疫吸附测定试剂盒Guinea pig ODC (Ornithine Decarboxylase) ELISA Kit
相关产品
详细地址
关注
拨打电话
留言咨询