供货周期: | 一周 |
品牌: | Abcam |
规格: | 0.1ml/100μg 0.2ml/200μg |
货号: | Ab-07379 |
CAS号: | 此产品无CAS编号 |
?丝氨酸/苏氨酸蛋白激酶MARK4抗体
『别名』MARK 4; MARK-4; MARK 4L; MARK L1; MARK4; MARK4 serine / threonine protein kinase; MARK4 serine/threonine protein kinase; MARK4_HUMAN; MARKL 1; MARKL1; MAP / microtubule affinity regulating kinase 4; MAP / microtubule affinity regulating kinase 4L; MAP/microtubule affinity regulating kinase 4; MAP/microtubule affinity regulating kinase like 1; MAP/microtubule affinity-regulating kinase 4; MAP/microtubule affinity-regulating kinase-like 1; Putative protein product of Nbla00650; FLJ90097; KIAA1860; mKIAA1860.
『浓度』丝氨酸/苏氨酸蛋白激酶MARK4抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
丝氨酸/苏氨酸蛋白激酶MARK4抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
丝氨酸/苏氨酸蛋白激酶MARK4抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
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EL1255c 豚鼠免疫球蛋白E Fc段受体I(FcεR I)酶联免疫吸附测定试剂盒 Guinea pig FcεR I (Receptor I for the Fc Fragment of IgE) ELISA Kit
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EL1000c 豚鼠纤维胶凝蛋白1(FCN1)酶联免疫吸附测定试剂盒 Guinea pig FCN1 (Ficolin 1) ELISA Kit
EL0804c 豚鼠IgA Fc片段受体(FcaR)酶联免疫吸附测定试剂盒 Guinea pig FcaR (Fc Fragment of IgA Receptor) ELISA Kit
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EL0468c 豚鼠衰老关键蛋白4(FBLN4)酶联免疫吸附测定试剂盒 Guinea pig FBLN4 (Fibulin 4) ELISA Kit
EL0648c 豚鼠纤维蛋白原(FBG)酶联免疫吸附测定试剂盒 Guinea pig FBG (Fibrinogen) ELISA Kit
EL0379c 豚鼠脂肪酸合酶(FASN)酶联免疫吸附测定试剂盒 Guinea pig FASN (Fatty Acid Synthase) ELISA Kit
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EL0859c 豚鼠Fas凋亡抑制分子3(FAIM3)酶联免疫吸附测定试剂盒 Guinea pig FAIM3 (Fas Apoptotic Inhibitory Molecule 3) ELISA Kit
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EL0264c 豚鼠脂肪酸结合蛋白3(FABP3)酶联免疫吸附测定试剂盒 Guinea pig FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) ELISA Kit
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EL0932c 豚鼠凝血因子Ⅱ(F2)酶联免疫吸附测定试剂盒 Guinea pig F2 (Coagulation Factor Ⅱ) ELISA Kit
EL0922c 豚鼠凝血因子ⅩⅢ(F13)酶联免疫吸附测定试剂盒 Guinea pig F13 (Coagulation Factor ⅩⅢ) ELISA Kit
EL1314c 豚鼠凝血因子Ⅻ(F12)酶联免疫吸附测定试剂盒 Guinea pig F12 (Coagulation Factor Ⅻ) ELISA Kit
EL0055c 豚鼠凝血因子XI(F11)酶联免疫吸附测定试剂盒 Guinea pig F11 (Coagulation Factor XI) ELISA Kit
EL1169c 豚鼠N端外显肽(Ext-N)酶联免疫吸附测定试剂盒 Guinea pig Ext-N (N-Terminal Extein) ELISA Kit
EL0575c 豚鼠外显肽(Extein)酶联免疫吸附测定试剂盒 Guinea pig Extein ELISA Kit
EL0822c 豚鼠外生骨疣蛋白1(EXT1)酶联免疫吸附测定试剂盒 Guinea pig EXT1 (Exostoses 1) ELISA Kit
EL0178c 豚鼠内皮素1(ET-1)酶联免疫吸附测定试剂盒 Guinea pig ET-1 (Endothelin 1) ELISA Kit
EL0813c 豚鼠表睾酮(ET)酶联免疫吸附测定试剂盒 Guinea pig ET (Epitestosterone) ELISA Kit
EL0883c 豚鼠红细胞刺激因子(ESF)酶联免疫吸附测定试剂盒 Guinea pig ESF (Erythropoiesis Stimulating Factor) ELISA Kit
EL0353c 豚鼠内皮抑制素(ES)酶联免疫吸附测定试剂盒 Guinea pig ES (Endostatin) ELISA Kit
EL0802c 豚鼠雌激素受体α(ERα)酶联免疫吸附测定试剂盒 Guinea pig ERα (Estrogen Receptor Alpha) ELISA Kit
EL0885c 豚鼠内质网核信号1(ERN1)酶联免疫吸附测定试剂盒 Guinea pig ERN1 (Endoplasmic Reticulum to Nucleus Signalling 1) ELISA Kit
EL0296c 豚鼠细胞外信号调节激酶3(ERK3)酶联免疫吸附测定试剂盒 Guinea pig ERK3 (Extracellular Signal Regulated Kinase 3) ELISA Kit
EL1176c 豚鼠细胞外信号调节激酶1(ERK1)酶联免疫吸附测定试剂盒 Guinea pig ERK1 (Extracellular Signal Regulated Kinase 1) ELISA Kit
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EL1075c 豚鼠剪切修复交叉互补基因1(ERCC1)酶联免疫吸附测定试剂盒 Guinea pig ERCC1 (Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1) ELISA Kit
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EL0604c 豚鼠促红细胞生成素(EPO)酶联免疫吸附测定试剂盒 Guinea pig EPO (Erythropoietin) ELISA Kit
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