鸡白介素1检测试剂盒代测

报价:面议
供货周期: 7天
品牌: Sigma-Aldrich
型号: 48/96孔
货号: Gu-20076
CAS号: 此产品无CAS号
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鸡白介素1检测试剂盒代测

我们免费代测,品牌供应,我们拥有三千多种酶联免疫吸附测定试剂盒,包含多种种属ELISA试剂盒,待测标本形式多样:有液体及半固体形式。

样本保存:

2-8℃冰箱最多2周

-20到-50℃的冰箱可以放3-6个月

鸡白介素1检测试剂盒代测-50℃一下的冰箱,可以放一年

样本收集:

血清样本用干燥管(红色)或者促凝管(黄色)收集

血浆用抗凝管,一般有EDTA抗凝(紫色)、枸橼酸钠抗凝(黑色或者蓝色)

血浆样本最好是不要用肝素(绿色)抗凝的试管收集

样本处理:

1)血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如出现沉淀,应再次离心。

2)血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次离心。

3)尿液:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。

4)细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。鸡白介素1检测试剂盒代测通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。

5)组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。

6)标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融.

7)不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。

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EIA(B004)-014439 犬RasGTP酶激活蛋白(RasGAP)ELISA Kit ,Products name: RasGAP ELISA Kit ,灵敏度: 0.93799999999999994ng/mL ,检测范围: 1.5629999999999999-100ng/mL

EIA(B004)-014440 犬ras同源物基因家族成员b(RHOB)ELISA Kit ,Products name: RHOB ELISA Kit ,灵敏度: 0.188ng/mL ,检测范围: 0.313-20ng/mL

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EIA(B004)-014445 犬S100B蛋白(S-100B)ELISA Kit ,Products name: S-100B ELISA Kit ,灵敏度: 9.375pg/mL ,检测范围: 15.625-1000pg/mL

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EIA(B004)-014448 犬S-100蛋白(S-100)ELISA Kit ,Products name: S-100 ELISA Kit ,灵敏度: 37.5pg/mL ,检测范围: 62.5-4000pg/mL

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鸡白介素1检测试剂盒代测由上海弘顺生物科技有限公司为您提供,货号Gu-20076,规格:48/96孔,CAS号:此产品无CAS号,如您想了解更多关于鸡白介素1检测试剂盒代测价格、鸡白介素1检测试剂盒代测结构式、批发、用途等信息,欢迎咨询。除供应鸡白介素1检测试剂盒代测外,还可为您提供其他等试剂,公司有专业的客户服务团队,是您值得信赖的合作伙伴,弘顺生物客户服务电话,售前、售后均可联系。
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