供货周期: | 一周 |
品牌: | Sigma-Aldrich |
规格: | 48/96孔 |
货号: | Hu-20228 |
CAS号: | 此产品无CAS号 |
人可溶性肿瘤坏死因子相关凋亡诱导配体ELISA试剂盒厂家
我们免费代测,品牌供应,我们拥有三千多种酶联免疫吸附测定试剂盒,包含多种种属ELISA试剂盒,待测标本形式多样:有液体及半固体形式。
样本保存:
2-8℃冰箱最多2周
-20到-50℃的冰箱可以放3-6个月
人可溶性肿瘤坏死因子相关凋亡诱导配体ELISA试剂盒厂家-50℃一下的冰箱,可以放一年
样本收集:
血清样本用干燥管(红色)或者促凝管(黄色)收集
血浆用抗凝管,一般有EDTA抗凝(紫色)、枸橼酸钠抗凝(黑色或者蓝色)
血浆样本最好是不要用肝素(绿色)抗凝的试管收集
样本处理:
1)血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如出现沉淀,应再次离心。
2)血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次离心。
3)尿液:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。
4)细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。人可溶性肿瘤坏死因子相关凋亡诱导配体ELISA试剂盒厂家通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
5)组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
6)标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融.
7)不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
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EIA(B004)-032172 人组织蛋白酶D(cath-D)ELISA Kit ,Products name: Human cath-D ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
EIA(B004)-032173 人组织蛋白酶D(cath-D)ELISA Kit ,Products name: Cath-D ELISA Kit ,灵敏度: 0.469ng/mL ,检测范围: 0.781-50ng/mL
EIA(B004)-032174 人组织蛋白酶D(CTSD)ELISA Kit ,Products name: Human CTSD ELISA Kit ,灵敏度: 9.375pg/mL ,检测范围: 15.625-1000pg/mL
EIA(B004)-032175 人组织蛋白酶D(CTSD)ELISA Kit ,Products name: CTSD ELISA Kit ,灵敏度: 0.150ng/mL ,检测范围: 0.25-16ng/mL
EIA(B004)-032176 人组织蛋白酶F(CTSF)ELISA Kit ,Products name: Human CTSF ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
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EIA(B004)-032178 人组织蛋白酶G(CTSG)ELISA Kit ,Products name: Human CTSG ELISA Kit ,灵敏度: 9.375pg/mL ,检测范围: 15.625-1000pg/mL
EIA(B004)-032179 人组织蛋白酶G自身抗体(CathGAb)ELISA Kit ,Products name: Human Cath G Ab ELISA Kit ,灵敏度: 0.375ng/mL ,检测范围: 0.625-40ng/mL
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EIA(B004)-032182 人组织蛋白酶K(cath-K)ELISA Kit ,Products name: Human Cathepsin K,cath-K ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
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EIA(B004)-032184 人组织蛋白酶K(cath-K)ELISA Kit ,Products name: Cath-K ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
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