供货周期: | 一周 |
品牌: | Abcam |
规格: | 0.1ml/100μg 0.2ml/200μg |
货号: | Ab-07022 |
CAS号: | 此产品无CAS编号 |
丝裂原活化蛋白激酶组织蛋白1抗体
『别名』MAPK organizer 1; MGC4238; Mitogen activated protein kinase organizer 1; Mitogen-activated protein kinase organizer 1; MORG 1; WD repeat domain-containing protein 83; wdr83; WDR83_HUMAN.
『浓度』丝裂原活化蛋白激酶组织蛋白1抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
丝裂原活化蛋白激酶组织蛋白1抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
丝裂原活化蛋白激酶组织蛋白1抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
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PAA166Ra01 胃蛋白酶原C(PGC)多克隆抗体 Polyclonal Antibody to Pepsinogen C (PGC)
PAA166Mu02 胃蛋白酶原C(PGC)多克隆抗体 Polyclonal Antibody to Pepsinogen C (PGC)
PAA165Bo01 胃蛋白酶原A(PGA)多克隆抗体 Polyclonal Antibody to Pepsinogen A (PGA)
PAA165Hu01 胃蛋白酶原A(PGA)多克隆抗体 Polyclonal Antibody to Pepsinogen A (PGA)
PAA165Ra01 胃蛋白酶原A(PGA)多克隆抗体 Polyclonal Antibody to Pepsinogen A (PGA)
PAA632Hu01 胃蛋白酶(PP)多克隆抗体 Polyclonal Antibody to Pepsin (PP)
PAA632Po01 胃蛋白酶(PP)多克隆抗体 Polyclonal Antibody to Pepsin (PP)
PAA632Hu02 胃蛋白酶(PP)多克隆抗体 Polyclonal Antibody to Pepsin (PP)
PAK411Hu01 正五聚蛋白3(PTX3)多克隆抗体 Polyclonal Antibody to Pentraxin 3, Long (PTX3)
PAK411Ra01 正五聚蛋白3(PTX3)多克隆抗体 Polyclonal Antibody to Pentraxin 3, Long (PTX3)
PAK411Mu01 正五聚蛋白3(PTX3)多克隆抗体 Polyclonal Antibody to Pentraxin 3, Long (PTX3)
PAM001Hu01 PDZ和LIM域蛋白1(PDLIM1)多克隆抗体 Polyclonal Antibody to PDZ And LIM Domain Protein 1 (PDLIM1)
PAM911Hu01 15kDa PCNA关联因子(PAF)多克隆抗体 Polyclonal Antibody to PCNA Associated Factor 15kDa (PAF)
PAB844Hu01 桩蛋白(Pax)多克隆抗体 Polyclonal Antibody to Paxillin (Pax)
PAB844Mu01 桩蛋白(Pax)多克隆抗体 Polyclonal Antibody to Paxillin (Pax)
PAB844Ra01 桩蛋白(Pax)多克隆抗体 Polyclonal Antibody to Paxillin (Pax)
PAK340Hu01 补缀同源物1(PTCH1)多克隆抗体 Polyclonal Antibody to Patched 1 (PTCH1)
PAK340Mu01 补缀同源物1(PTCH1)多克隆抗体 Polyclonal Antibody to Patched 1 (PTCH1)
PAH868Hu01 含Patatin样磷脂酶域蛋白3(PNPLA3)多克隆抗体 Polyclonal Antibody to Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3)
PAH868Ra01 含Patatin样磷脂酶域蛋白3(PNPLA3)多克隆抗体 Polyclonal Antibody to Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3)
PAH869Ra01 含Patatin样磷脂酶域蛋白2(PNPLA2)多克隆抗体 Polyclonal Antibody to Patatin Like Phospholipase Domain Containing Protein 2 (PNPLA2)
PAG439Hu01 小白蛋白(PVALB)多克隆抗体 Polyclonal Antibody to Parvalbumin (PVALB)
PAG439Ra01 小白蛋白(PVALB)多克隆抗体 Polyclonal Antibody to Parvalbumin (PVALB)
PAL059Hu01 帕金森氏病蛋白7(PARK7)多克隆抗体 Polyclonal Antibody to Parkinson Disease Protein 7 (PARK7)
PAL059Mu01 帕金森氏病蛋白7(PARK7)多克隆抗体 Polyclonal Antibody to Parkinson Disease Protein 7 (PARK7)
PAL059Ra01 帕金森氏病蛋白7(PARK7)多克隆抗体 Polyclonal Antibody to Parkinson Disease Protein 7 (PARK7)
PAL060Hu01 帕金森氏病蛋白2(PARK2)多克隆抗体 Polyclonal Antibody to Parkinson Disease Protein 2 (PARK2)
PAA819Hu01 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 Polyclonal Antibody to Parathyroid Hormone Related Protein (PTHrP)
PAA819Mu01 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 Polyclonal Antibody to Parathyroid Hormone Related Protein (PTHrP)
PAA819Ra01 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 Polyclonal Antibody to Parathyroid Hormone Related Protein (PTHrP)
PAA819Ca01 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 Polyclonal Antibody to Parathyroid Hormone Related Protein (PTHrP)
PAA819Ga01 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 Polyclonal Antibody to Parathyroid Hormone Related Protein (PTHrP)
PAA819Bo01 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 Polyclonal Antibody to Parathyroid Hormone Related Protein (PTHrP)
PAA819Mu02 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 Polyclonal Antibody to Parathyroid Hormone Related Protein (PTHrP)
PAA819Hu02 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 Polyclonal Antibody to Parathyroid Hormone Related Protein (PTHrP)
PAA474Hu01 甲状旁腺素受体2(PTHR2)多克隆抗体 Polyclonal Antibody to Parathyroid Hormone Receptor 2 (PTHR2)
PAA474Ra01 甲状旁腺素受体2(PTHR2)多克隆抗体 Polyclonal Antibody to Parathyroid Hormone Receptor 2 (PTHR2)
PAC743Hu01 甲状旁腺素受体1(PTHR1)多克隆抗体 Polyclonal Antibody to Parathyroid Hormone Receptor 1 (PTHR1)
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PAA866Ca01 甲状旁腺激素(PTH)多克隆抗体 Polyclonal Antibody to Parathyroid Hormone (PTH)
PAA866Ga01 甲状旁腺激素(PTH)多克隆抗体 Polyclonal Antibody to Parathyroid Hormone (PTH)
PAA866Hu01 甲状旁腺激素(PTH)多克隆抗体 Polyclonal Antibody to Parathyroid Hormone (PTH)
PAA866Mu01 甲状旁腺激素(PTH)多克隆抗体 Polyclonal Antibody to Parathyroid Hormone (PTH)
PAA866Po01 甲状旁腺激素(PTH)多克隆抗体 Polyclonal Antibody to Parathyroid Hormone (PTH)
PAA866Ra01 甲状旁腺激素(PTH)多克隆抗体 Polyclonal Antibody to Parathyroid Hormone (PTH)
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PAD220Mu01 胸腺旁腺素(PTMS)多克隆抗体 Polyclonal Antibody to Parathymosin (PTMS)
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