供货周期: | 一周 |
品牌: | Abcam |
规格: | 0.1ml/100μg 0.2ml/200μg |
货号: | Ab-07025 |
CAS号: | 此产品无CAS编号 |
突变的黑色素瘤相关抗原1抗体
『别名』EXPAND1; FLJ14868; FLJ22283; HSPC211; Melanoma associated antigen (mutated) 1; Melanoma ubiquitous mutated protein; MGC131891; MGC163315; MUM 1; MUM-1; MUM1; MUM1_HUMAN; Mutated melanoma associated antigen 1; Mutated melanoma-associated antigen 1; Protein expandere; PWWP domain containing protein MUM1; PWWP domain-containing protein MUM1.
『浓度』突变的黑色素瘤相关抗原1抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
突变的黑色素瘤相关抗原1抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
突变的黑色素瘤相关抗原1抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAA110Hu01 抑瘤素M(OSM)多克隆抗体 Polyclonal Antibody to Oncostatin M (OSM)
PAA110Mu01 抑瘤素M(OSM)多克隆抗体 Polyclonal Antibody to Oncostatin M (OSM)
PAA110Ra01 抑瘤素M(OSM)多克隆抗体 Polyclonal Antibody to Oncostatin M (OSM)
PAA110Mu02 抑瘤素M(OSM)多克隆抗体 Polyclonal Antibody to Oncostatin M (OSM)
PAB729Hu01 癌蛋白诱导转录物3(OIT3)多克隆抗体 Polyclonal Antibody to Oncoprotein Induced Transcript 3 (OIT3)
PAB729Ra01 癌蛋白诱导转录物3(OIT3)多克隆抗体 Polyclonal Antibody to Oncoprotein Induced Transcript 3 (OIT3)
PAH506Hu01 癌调蛋白(OCM)多克隆抗体 Polyclonal Antibody to Oncomodulin (OCM)
PAH506Ra01 癌调蛋白(OCM)多克隆抗体 Polyclonal Antibody to Oncomodulin (OCM)
PAH506Mu01 癌调蛋白(OCM)多克隆抗体 Polyclonal Antibody to Oncomodulin (OCM)
PAC690Hu01 少突胶质细胞系转录因子2(OLIG2)多克隆抗体 Polyclonal Antibody to Oligodendrocyte Lineage Transcription Factor 2 (OLIG2)
PAS975Hu01 嗅觉受体1J2(OR1J2)多克隆抗体 Polyclonal Antibody to Olfactory Receptor 1J2 (OR1J2)
PAA162Bo01 嗅素4(OLFM4)多克隆抗体 Polyclonal Antibody to Olfactomedin 4 (OLFM4)
PAA162Hu01 嗅素4(OLFM4)多克隆抗体 Polyclonal Antibody to Olfactomedin 4 (OLFM4)
PAA162Mu01 嗅素4(OLFM4)多克隆抗体 Polyclonal Antibody to Olfactomedin 4 (OLFM4)
PAA162Ra01 嗅素4(OLFM4)多克隆抗体 Polyclonal Antibody to Olfactomedin 4 (OLFM4)
PAA162Mu02 嗅素4(OLFM4)多克隆抗体 Polyclonal Antibody to Olfactomedin 4 (OLFM4)
PAK276Ra01 O-岩藻糖肽3-β-N-乙酰氨基葡萄糖转移酶(RFNG)多克隆抗体 Polyclonal Antibody to O-Fucosylpeptide-3-Beta-N-Acetylglucosaminyltransferase (RFNG)
PAC686Hu01 气味结合蛋白2A(OBP2A)多克隆抗体 Polyclonal Antibody to Odorant Binding Protein 2A (OBP2A)
PAA424Mu01 八聚体结合转录因子4(OCT4)多克隆抗体 Polyclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4)
PAA424Ra01 八聚体结合转录因子4(OCT4)多克隆抗体 Polyclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4)
PAA424Mu02 八聚体结合转录因子4(OCT4)多克隆抗体 Polyclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4)
PAA424Hu01 八聚体结合转录因子4(OCT4)多克隆抗体 Polyclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4)
PAB554Hu01 八聚体结合转录因子2(OCT2)多克隆抗体 Polyclonal Antibody to Octamer Binding Transcription Factor 2 (OCT2)
PAB554Ra01 八聚体结合转录因子2(OCT2)多克隆抗体 Polyclonal Antibody to Octamer Binding Transcription Factor 2 (OCT2)
PAB553Hu01 八聚体结合转录因子1(OCT1)多克隆抗体 Polyclonal Antibody to Octamer Binding Transcription Factor 1 (OCT1)
PAB553Mu01 八聚体结合转录因子1(OCT1)多克隆抗体 Polyclonal Antibody to Octamer Binding Transcription Factor 1 (OCT1)
PAU428Hu01 含密封蛋白/ELL域蛋白1(OCEL1)多克隆抗体 Polyclonal Antibody to Occludin/ELL Domain Containing Protein 1 (OCEL1)
PAU428Mu01 含密封蛋白/ELL域蛋白1(OCEL1)多克隆抗体 Polyclonal Antibody to Occludin/ELL Domain Containing Protein 1 (OCEL1)
PAC228Hu01 密封蛋白(OCLN)多克隆抗体 Polyclonal Antibody to Occludin (OCLN)
PAC228Mu01 密封蛋白(OCLN)多克隆抗体 Polyclonal Antibody to Occludin (OCLN)
PAC228Ra01 密封蛋白(OCLN)多克隆抗体 Polyclonal Antibody to Occludin (OCLN)
PAC228Mu02 密封蛋白(OCLN)多克隆抗体 Polyclonal Antibody to Occludin (OCLN)
PAC027Hu01 O-6-甲基鸟嘌呤DNA甲基转移酶(MGMT)多克隆抗体 Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)
PAC027Ra01 O-6-甲基鸟嘌呤DNA甲基转移酶(MGMT)多克隆抗体 Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)
PAJ934Hu01 Numb同源物(NUMB)多克隆抗体 Polyclonal Antibody to Numb Homolog (NUMB)
PAJ934Mu01 Numb同源物(NUMB)多克隆抗体 Polyclonal Antibody to Numb Homolog (NUMB)
PAK296Hu01 含核苷酸结合寡聚化域蛋白1(NOD1)多克隆抗体 Polyclonal Antibody to Nucleotide Binding Oligomerization Domain Containing Protein 1 (NOD1)
PAH571Hu01 核小体装配蛋白1样1(NAP1L1)多克隆抗体 Polyclonal Antibody to Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1)
PAH508Hu01 核氧化还原蛋白(NXN)多克隆抗体 Polyclonal Antibody to Nucleoredoxin (NXN)
PAH508Mu01 核氧化还原蛋白(NXN)多克隆抗体 Polyclonal Antibody to Nucleoredoxin (NXN)
PAB196Hu01 98kDa核孔蛋白(NUP98)多克隆抗体 Polyclonal Antibody to Nucleoporin 98kDa (NUP98)
PAB196Mu01 98kDa核孔蛋白(NUP98)多克隆抗体 Polyclonal Antibody to Nucleoporin 98kDa (NUP98)
PAB198Ra01 88kDa核孔蛋白(NUP88)多克隆抗体 Polyclonal Antibody to Nucleoporin 88kDa (NUP88)
PAB198Hu01 88kDa核孔蛋白(NUP88)多克隆抗体 Polyclonal Antibody to Nucleoporin 88kDa (NUP88)
PAB198Mu01 88kDa核孔蛋白(NUP88)多克隆抗体 Polyclonal Antibody to Nucleoporin 88kDa (NUP88)
PAB110Hu01 85kDa核孔蛋白(NUP85)多克隆抗体 Polyclonal Antibody to Nucleoporin 85kDa (NUP85)
PAB110Mu01 85kDa核孔蛋白(NUP85)多克隆抗体 Polyclonal Antibody to Nucleoporin 85kDa (NUP85)
PAC257Hu01 62kDa核孔蛋白(NUP62)多克隆抗体 Polyclonal Antibody to Nucleoporin 62kDa (NUP62)
PAB101Mu01 50kDa核孔蛋白(NUP50)多克隆抗体 Polyclonal Antibody to Nucleoporin 50kDa (NUP50)
PAB101Hu01 50kDa核孔蛋白(NUP50)多克隆抗体 Polyclonal Antibody to Nucleoporin 50kDa (NUP50)
PAB101Ra01 50kDa核孔蛋白(NUP50)多克隆抗体 Polyclonal Antibody to Nucleoporin 50kDa (NUP50)
PAB087Hu01 35kDa核孔蛋白突变的黑色素瘤相关抗原1抗体(NUP35)多克隆抗体 Polyclonal Antibody to Nucleoporin 35kDa (NUP35)
PAB169Mu01 214kDa核孔蛋白(NUP214)多克隆抗体 Polyclonal Antibody to Nucleoporin 214kDa (NUP214)
PAB421Mu01 210kDa核孔蛋白(NUP210)多克隆抗体 Polyclonal Antibody to Nucleoporin 210kDa (NUP210)
PAB421Hu01 210kDa核孔蛋白(NUP210)多克隆抗体 Polyclonal Antibody to Nucleoporin 210kDa (NUP210)
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