供货周期: | 一周 |
品牌: | Abcam |
规格: | 0.1ml/100μg 0.2ml/200μg |
货号: | Ab-07036 |
CAS号: | 此产品无CAS编号 |
生长激素诱导跨膜蛋白抗体(真皮乳头源性蛋白2)
『别名』Dermal papilla derived protein 2; Dermal papilla-derived protein 2; DERP2; Ghitm; GHITM_HUMAN; Growth hormone inducible transmembrane protein; Growth hormone-inducible transmembrane protein; HSPC282; MICS1; Mitochondrial morphology and cristae structure 1; My021; PTD010; TMBIM5; Transmembrane BAX inhibitor motif containing 5; Transmembrane BAX inhibitor motif containing protein 5; Transmembrane BAX inhibitor motif-containing protein 5.
『浓度』生长激素诱导跨膜蛋白抗体(真皮乳头源性蛋白2)1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
生长激素诱导跨膜蛋白抗体(真皮乳头源性蛋白2)洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
生长激素诱导跨膜蛋白抗体(真皮乳头源性蛋白2)其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAA539Mu02 髓鞘碱性蛋白(MBP)多克隆抗体 Polyclonal Antibody to Myelin Basic Protein (MBP)
PAA539Rb51 髓鞘碱性蛋白(MBP)多克隆抗体 Polyclonal Antibody to Myelin Basic Protein (MBP)
PAA539Hu02 髓鞘碱性蛋白(MBP)多克隆抗体 Polyclonal Antibody to Myelin Basic Protein (MBP)
PAA422Hu01 髓鞘关联糖蛋白(MAG)多克隆抗体 Polyclonal Antibody to Myelin Associated Glycoprotein (MAG)
PAA422Ra01 髓鞘关联糖蛋白(MAG)多克隆抗体 Polyclonal Antibody to Myelin Associated Glycoprotein (MAG)
PAA422Hu02 髓鞘关联糖蛋白(MAG)多克隆抗体 Polyclonal Antibody to Myelin Associated Glycoprotein (MAG)
PAH676Hu01 MYC关联因子X(MAX)多克隆抗体 Polyclonal Antibody to MYC Associated Factor X (MAX)
PAJ746Hu01 MutY同源物(MUTYH)多克隆抗体 Polyclonal Antibody to MutY Homolog (MUTYH)
PAJ742Hu01 MutL同源物1(MLH1)多克隆抗体 Polyclonal Antibody to MutL Homolog 1 (MLH1)
PAE270Hu01 骨骼肌特异性受体酪氨酸激酶(MUSK)多克隆抗体 Polyclonal Antibody to Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK)
PAE270Mu01 骨骼肌特异性受体酪氨酸激酶(MUSK)多克隆抗体 Polyclonal Antibody to Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK)
PAE270Mu02 骨骼肌特异性受体酪氨酸激酶(MUSK)多克隆抗体 Polyclonal Antibody to Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK)
PAC248Hu01 多发性内分泌腺瘤Ⅰ型蛋白(MEN1)多克隆抗体 Polyclonal Antibody to Multiple Endocrine Neoplasia I (MEN1)
PAF593Hu01 多聚蛋白2(MMRN2)多克隆抗体 Polyclonal Antibody to Multimerin 2 (MMRN2)
PAF593Mu01 多聚蛋白2(MMRN2)多克隆抗体 Polyclonal Antibody to Multimerin 2 (MMRN2)
PAF593Mu02 多聚蛋白2(MMRN2)多克隆抗体 Polyclonal Antibody to Multimerin 2 (MMRN2)
PAC622Hu01 多聚蛋白1(MMRN1)多克隆抗体 Polyclonal Antibody to Multimerin 1 (MMRN1)
PAC622Ra01 多聚蛋白1(MMRN1)多克隆抗体 Polyclonal Antibody to Multimerin 1 (MMRN1)
PAB068Hu01 多药耐药关联蛋白1(MRP1)多克隆抗体 Polyclonal Antibody to Multidrug Resistance Associated Protein 1 (MRP1)
PAB068Mu01 多药耐药关联蛋白1(MRP1)多克隆抗体 Polyclonal Antibody to Multidrug Resistance Associated Protein 1 (MRP1)
PAB068Ra01 多药耐药关联蛋白1(MRP1)多克隆抗体 Polyclonal Antibody to Multidrug Resistance Associated Protein 1 (MRP1)
PAB521Hu01 黏膜地址素细胞黏附分子(MAdCAM1)多克隆抗体 Polyclonal Antibody to Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1)
PAB521Mu01 黏膜地址素细胞黏附分子(MAdCAM1)多克隆抗体 Polyclonal Antibody to Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1)
PAB521Mu02 黏膜地址素细胞黏附分子(MAdCAM1)多克隆抗体 Polyclonal Antibody to Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1)
PAB573Mu01 粘膜关联上皮趋化因子(MEC)多克隆抗体 Polyclonal Antibody to Mucosae Associated Epithelia Chemokine (MEC)
PAB573Hu01 粘膜关联上皮趋化因子(MEC)多克隆抗体 Polyclonal Antibody to Mucosae Associated Epithelia Chemokine (MEC)
PAB573Ra01 粘膜关联上皮趋化因子(MEC)多克隆抗体 Polyclonal Antibody to Mucosae Associated Epithelia Chemokine (MEC)
PAB808Hu01 粘蛋白7(MUC7)多克隆抗体 Polyclonal Antibody to Mucin 7, Secreted (MUC7)
PAB808Bo01 粘蛋白7(MUC7)多克隆抗体 Polyclonal Antibody to Mucin 7, Secreted (MUC7)
PAB489Hu01 粘蛋白6(MUC6)多克隆抗体 Polyclonal Antibody to Mucin 6 (MUC6)
PAB489Mu01 粘蛋白6(MUC6)多克隆抗体 Polyclonal Antibody to Mucin 6 (MUC6)
PAB489Bo01 粘蛋白6(MUC6)多克隆抗体 Polyclonal Antibody to Mucin 6 (MUC6)
PAA684Hu01 粘蛋白5B(MUC5B)多克隆抗体 Polyclonal Antibody to Mucin 5 Subtype B (MUC5B)
PAA684Mu01 粘蛋白5B(MUC5B)多克隆抗体 Polyclonal Antibody to Mucin 5 Subtype B (MUC5B)
PAA684Bo01 粘蛋白5B(MUC5B)多克隆抗体 Polyclonal Antibody to Mucin 5 Subtype B (MUC5B)
PAA684Mu02 粘蛋白5B(MUC5B)多克隆抗体 Polyclonal Antibody to Mucin 5 Subtype B (MUC5B)
PAA684Ra01 粘蛋白5B(MUC5B)多克隆抗体 Polyclonal Antibody to Mucin 5 Subtype B (MUC5B)
PAA684Ra02 粘蛋白5B(MUC5B)多克隆抗体 Polyclonal Antibody to Mucin 5 Subtype B (MUC5B)
PAA684Ra03 粘蛋白5B(MUC5B)多克隆抗体 Polyclonal Antibody to Mucin 5 Subtype B (MUC5B)
PAA684Bo03 粘蛋白5B(MUC5B)多克隆抗体 Polyclonal Antibody to Mucin 5 Subtype B (MUC5B)
PAA756Hu01 粘蛋白5AC(MUC5AC)多克隆抗体 Polyclonal Antibody to Mucin 5 Subtype AC (MUC5AC)
PAA756Mu01 粘蛋白5AC(MUC5AC)多克隆抗体 Polyclonal Antibody to Mucin 5 Subtype AC (MUC5AC)
PAA756Ra01 粘蛋白5AC(MUC5AC)多克隆抗体 Polyclonal Antibody to Mucin 5 Subtype AC (MUC5AC)
PAA756Bo01 粘蛋白5AC(MUC5AC)多克隆抗体 Polyclonal Antibody to Mucin 5 Subtype AC (MUC5AC)
PAA756Mu02 粘蛋白5AC(MUC5AC)多克隆抗体 Polyclonal Antibody to Mucin 5 Subtype AC (MUC5AC)
PAA756Mu03 粘蛋白5AC(MUC5AC)多克隆抗体 Polyclonal Antibody to Mucin 5 Subtype AC (MUC5AC)
PAB034Hu01 粘蛋白4(MUC4)多克隆抗体 Polyclonal Antibody to Mucin 4 (MUC4)
PAB034Mu01 粘蛋白4(MUC4)多克隆抗体 Polyclonal Antibody to Mucin 4 (MUC4)
PAB034Ra01 粘蛋白4(MUC4)多克隆抗体 Polyclonal Antibody to Mucin 4 (MUC4)
PAB034Bo01 粘蛋白4(MUC4)多克隆抗体 Polyclonal Antibody to Mucin 4 (MUC4)
PAB034Mu02 粘蛋白4(MUC4)多克隆抗体 Polyclonal Antibody to Mucin 4 (MUC4)
PAP416Hu01 粘蛋白3B生长激素诱导跨膜蛋白抗体(真皮乳头源性蛋白2)(MUC3B)多克隆抗体 Polyclonal Antibody to Mucin 3B, Cell Surface Associated (MUC3B)
PAB031Hu01 粘蛋白3(MUC3)多克隆抗体 Polyclonal Antibody to Mucin 3 (MUC3)
PAB031Mu01 粘蛋白3(MUC3)多克隆抗体 Polyclonal Antibody to Mucin 3 (MUC3)
PAB031Ra01 粘蛋白3(MUC3)多克隆抗体 Polyclonal Antibody to Mucin 3 (MUC3)
PAB031Bo01 粘蛋白3(MUC3)多克隆抗体 Polyclonal Antibody to Mucin 3 (MUC3)
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