供货周期: | 7天 |
品牌: | Sigma-Aldrich |
型号: | 48/96孔 |
货号: | Hu-20327 |
CAS号: | 此产品无CAS号 |
人丙酮酸脱氢酶E1ELISA试剂盒厂家
我们免费代测,品牌供应,我们拥有三千多种酶联免疫吸附测定试剂盒,包含多种种属ELISA试剂盒,待测标本形式多样:有液体及半固体形式。
样本保存:
2-8℃冰箱最多2周
-20到-50℃的冰箱可以放3-6个月
人丙酮酸脱氢酶E1ELISA试剂盒厂家-50℃一下的冰箱,可以放一年
样本收集:
血清样本用干燥管(红色)或者促凝管(黄色)收集
血浆用抗凝管,一般有EDTA抗凝(紫色)、枸橼酸钠抗凝(黑色或者蓝色)
血浆样本最好是不要用肝素(绿色)抗凝的试管收集
样本处理:
1)血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如出现沉淀,应再次离心。
2)血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次离心。
3)尿液:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。
4)细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。人丙酮酸脱氢酶E1ELISA试剂盒厂家通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
5)组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
6)标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融.
7)不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
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EIA(B004)-037535 兔血管内皮生长因子受体2(VEGFR-2/KDR)ELISA Kit ,Products name: Rabbit VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2) ELISA Kit ,灵敏度: 0.93799999999999994ng/mL ,检测范围: 1.5629999999999999-100ng/mL
EIA(B004)-037536 兔血管内皮生长因子受体3(VEGFR 3)ELISA Kit ,Products name: VEGFR 3 ELISA Kit ,灵敏度: 0.188ng/mL ,检测范围: 0.313-20ng/mL
EIA(B004)-037537 兔血管内皮细胞蛋白C受体(PROCR)ELISA Kit ,Products name: PROCR ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-037538 兔血管内皮细胞生长因子(VEGF)ELISA Kit ,Products name: VEGF ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-037539 兔血管内皮细胞生长因子(VEGF)ELISA Kit ,Products name: Rabbit Vascular Endothelial cell Growth Factor,VEGF ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-037540 兔血管内皮细胞生长因子(VEGF)ELISA Kit ,Products name: Rabbit Vascular Endothelial cell Growth Factor,VEGF ELISA Kit ,灵敏度: 0.46899999999999997ng/mL ,检测范围: 0.78100000000000003-50ng/mL
EIA(B004)-037541 兔血管内皮细胞生长因子(VEGF)ELISA Kit ,Products name: VEGF ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-037542 兔血管内皮细胞生长因子(VEGF)ELISA Kit ,Products name: VEGF ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-037543 兔血管内皮细胞生长因子B(VEGF-B)ELISA Kit ,Products name: VEGF-B ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-037544 兔血管内皮细胞生长因子D(VEGF.D)ELISA Kit ,Products name: VEGF.D ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-037545 兔血管内皮细胞生长因子受体1(VEGFR-1/Flt1)ELISA Kit ,Products name: VEGFR-1/Flt1 ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
EIA(B004)-037546 兔血管内皮细胞粘附分子1(CD106)ELISA Kit ,Products name: Rabbit Vascuolar cell adhesion molecule 1,VCAM-1 ELISA Kit ,灵敏度: 0.188ng/mL ,检测范围: 0.313-20ng/mL
EIA(B004)-037547 兔血管内皮细胞粘附分子1(VCAM-1)ELISA Kit ,Products name: Rabbit Vascuolar cell adhesion molecule 1,VCAM-1 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-037548 兔血管内皮细胞粘附分子1(VCAM-1)ELISA Kit ,Products name: VCAM-1 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-037549 兔血管内皮细胞粘附分子1(VCAM-1)ELISA Kit ,Products name: VCAM-1 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-037550 兔血管内皮细胞粘附分子1(VCAM-1/CD106)ELISA Kit ,Products name: Rabbit Vascuolar cell adhesion molecule 1,VCAM-1 ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
EIA(B004)-037551 兔血管内皮细胞粘附分子1(VCAM-1/CD106)ELISA Kit ,Products name: VCAM-1/CD106 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-037552 兔血管内皮抑素抗体(ES-Ab)ELISA Kit ,Products name: ES-Ab ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-037553 兔血管生成素2(Ang 2)ELISA Kit ,Products name: Ang 2 ELISA Kit ,灵敏度: 56.25pg/mL ,检测范围: 93.75-6000pg/mL
EIA(B004)-037554 兔血管生成素2(ANG-2)ELISA Kit ,Products name: Rabbit Angiopoietin 2,ANG-2 ELISA Kit ,灵敏度: 18.75pg/mL ,检测范围: 31.25-2000pg/mL
EIA(B004)-037555 兔血管生成素2(ANG-2)ELISA Kit ,Products name: Rabbit Angiopoietin 2,ANG-2 ELISA Kit ,灵敏度: 0.93799999999999994ng/mL ,检测范围: 1.5629999999999999-100ng/mL
EIA(B004)-037556 兔血管生成素2(ANG-2)ELISA Kit ,Products name: ANG-2 ELISA Kit ,灵敏度: 56.25pg/mL ,检测范围: 93.75-6000pg/mL
EIA(B004)-037557 兔血管生成素2(ANGPT2)ELISA Kit ,Products name: ANGPT2 ELISA Kit ,灵敏度: 0.93799999999999994U/mL ,检测范围: 1.5629999999999999-100U/mL
EIA(B004)-037558 兔血管生成素4(ANG4)ELISA Kit ,Products name: ANG4 ELISA Kit ,灵敏度: 1.875ng/mL ,检测范围: 3.125-200ng/mL
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EIA(B004)-037571 兔血管性血友病因子/瑞斯托霉素辅因子(VWF)ELISA Kit ,Products name: VWF ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
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