大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)ELISA Kit

 

大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)ELISA Kit

产品类型:

ELISA Kit

产品名称:

大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)ELISA Kit

英文名称:

Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit

大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)ELISA Kit

规格:

96T

大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)ELISA Kit

种属:

Rat

待测物名称:

tartrate-resistant acid phosphatase 5b,TRACP-5b

缩写:

TRACP-5b

检测范围:

0.312 mIU/ml-20 mIU/ml

灵敏度:

0.078 mIU/ml

反应时间:

1-5h

所需样本体积:

50-100ul

检测波长:

450 nm

大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)ELISA Kit

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRACP-5b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRACP-5b present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRACP-5b is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRACP-5b bound in the initial step. The color development is stopped and the intensity of the color is measured.

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