CD 溶解酵素的CD温度波长扫描

2015/09/25   下载量： 6 次

方案摘要

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应用领域 生物产业
检测样本 生物发酵
检测项目
参考标准 JIST

CD spectrum and fluorescence spectrum are used in a complementary manner in the structural analysis of the proteins. The thermodynamic parameters of the protein obtained by the measurement with variable temperature provide the important knowledge for the thermal stability of the protein. The Temperature/Wavelength Scan program for JASCO CD spectrometers allows to obtain the data of CD with the temperature change for the protein and DNA required to calculate the denaturation temperature (Tm), enthalpy change (DH), and entropy change (DS). In addition such program also enables temperature interval measurements of CD and fluorescence spectra. The obtained data can be processed on the [Spectrum Analysis] and [Interval Analysis] program, which also enables to show the 3D representation. The secondary structure analysis and the calculation of thermodynamic parameters of the protein can be implemented by using optional [Thermal Denaturation Analysis] and [Protein SSE] program. This report introduces the measurement of Lysozyme (0.03 mg/mL) using Temperature/Wavelength Scan program as an example. Key words: CD spectra measurement, Fluorescence spectra measurement, Melting temperature, Proteins

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CD spectrum and fluorescence spectrum are used in a complementary manner in the structural analysis of the proteins. The thermodynamic parameters of the protein obtained by the measurement with variable temperature provide the important knowledge for the thermal stability

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下一篇 CD 评价多种蛋白质的二级结构的(SSE)程序

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佳士科商贸有限公司
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