林木耐旱创新科研平台： 转录因子PdNF-YB21调控杨树根系生长影响抗旱

NMT作为生命科学底层核心技术，是建立活体创新科研平台的必备技术。2005年~2020年，NMT已扎根中国15年。2020年，中国NMT销往瑞士苏黎世大学，正式打开欧洲市场。

研究使用平台：NMT林木耐旱创新科研平台

期刊：New Phytologist
主题：转录因子PdNF-YB21调控杨树根系生长并影响抗旱性

标题：The root-specific NF-Y family transcription   factor, PdNF-YB21, positively regulates root growth and drought   resistance by ABA-mediated IAA transport in Populus

影响因子：7.299
检测指标：H+

检测样品：杨树根

H+流实验处理方法：

杨树茎段在Hoagland营养液/Hoagland营养液+0.1μM ABA/Hoagland营养液+10μM FLU培养30天后检测根。
H+流实验测试液成份：未写明

作者：北京林业大学夏新莉、周扬颜

中文摘要（谷歌机翻）

根系生长控制在植物对干旱胁迫的适应中起着重要作用，但是这种控制的潜在分子机制仍然很难捉摸。

在此，从胡杨中分离出根特异性核因子Y（NF-Y）转录因子PdNF-YB21。PdNF-YB21的功能机制通过各种形态，生理，分子，生化和光谱学技术进行了表征。

杨树中PdNF-YB21的过表达促进木质部血管高度木质化和扩大的根系生长，从而增加了抗旱性。相反，CRISPR  /   Cas9介导的杨树突变体nf-yb21表现出降低的根系生长和抗旱性。PdNF-YB21与B3域转录因子PdFUSCA3（PdFUS3）相互作用。PdFUS3直接激活ABA合成关键基因PdNCED3的启动子，导致水分缺乏的杨树根ABA含量显着增加。杨NF-YB21和FUS3的共表达显着增强了PdNCED3的表达。

此外，ABA促进了IAA在根尖的转运，最终增加了根的生长和抗旱性。

英文摘要

Root growth control plays an important role in plant adaptation to drought stress, but the underlying molecular mechanisms of  this control remain largely elusive.

Here, a root-specific  nuclear factor Y (NF-Y) transcription factor PdNF-YB21 was isolated from  Populus. The functional mechanism of PdNF-YB21 was characterized by  various morphological, physiological, molecular, biochemical, and  spectroscopy techniques.

Overexpression of PdNF-YB21 in poplar  promoted root growth with highly lignified and enlarged xylem vessels,  resulting in increased drought resistance. In contrast,  CRISPR/Cas9-mediated poplar mutant nf-yb21 exhibited reduced root growth  and drought resistance. PdNF-YB21 interacted with PdFUSCA3 (PdFUS3), a  B3 domain transcription factor. PdFUS3 directly activated the promoter  of the ABA synthesis key gene PdNCED3, resulting in a significant  increase in root ABA content in poplars subjected to water deficit.  Coexpression of poplar NF-YB21 and FUS3 significantly enhanced the  expression of PdNCED3.

Furthermore, ABA promoted IAA transport in root tips, which ultimately increased root growth and drought resistance.