仪器对比
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细胞分析系统
HoloMonitor M4
M4激光全息细胞成像分析系统，配备和M3同样强大的细胞图像分析软件，实现在细胞正常生
长环境下的非侵入，非标记活细胞成像及分析。
M4仪器非常精巧，可以放置在培养箱中，实现非常完美的Timelapse（延时成像）分析。同时，
测量并分析细胞成像，细胞计数，细胞面积和体积等形态学参数。
M4性能优势
*实时3D成像
*直接测量贴壁细胞
*非标记，无需染色，无光毒性
*细胞面积和体积分析
*长期time-lapse成像分析
*细胞跟踪功能
*体积小巧，可直接放在培养箱中
下面一组Time-lapse图片展示了人巨噬细胞吞噬酵母细胞时形态的改变
如下一组Time-lapse图片展示了人MCF-10A乳腺细胞（30分钟间隔拍摄）
技术参数：
图像类型: 定量相位图像
CCD相机: 1280×1024 像素, 8 or 10 bits (全息图)
光源:单色激光 (全息 模式)
物镜: 20x全息
计算机: 处理器 Intel Core i7, Memory 6GB, Hard drive 1TB, Graphics NVidia
1GB, Screen 1920x1080, Full HD, Windows 7 Professional
软件: Holostudio™ 2.3图像和细胞分析软件
系统性能
工作距离: 1.2 mm
数字聚焦范围:最大 50×景深
抓拍时间: 低于 4μs/图像
立体取样: 1024 × 1024 pixels (全息图)
捕获速度: 4fps (1024 × 1024 像素)
重建速度: 4fps (512 × 512 像素), 1 fps (1024 × 1024像素)
样品照度: ~0.1 mW/cm
样品容器高度: 最大45mm
电压: 100-249 VAC - 47/63 Hz 功率(w/o PC): max. 80 W
体积(L × W × H): 250 × 160 × 180 mm 重量: 2.5 kg
自2008年起，瑞典Phiab公司的激光全息分析系统就有大量用户文献发表，特挑选近三年文献与大家分享。
2014
Live-cell mass profiling: an emerging approach in quantitative biophysics Nature Methods
The role of pi3k/akt-related pip5k1α and the discovery of its selective inhibitor for treatment of advanced prostate cancer PNAS
Quantitative stain-free and continuous multimodal monitoring of wound healing in vitro with digital holographic microscopy PLoS ONE
Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in l929 cells PLoS ONE
Edaravone protects against methylglyoxal-induced barrier damage in human brain endothelial cells PLoS ONE
Environmental and toxicological impacts of glyphosate with its formulating adjuvant International Journal of Agricultural, Biosystems Science and Engineering
Prevention and treatment of colon cancer by peroral administration of hamlet Gut
2015
From immobilized cells to motile cells on a bed-of-nails: effects of vertical nanowire array density on cell behavior Scientific Reports 2015
Enhanced cytotoxicity of folic acid-targeted liposomes co-loaded with c6 ceramide and doxorubicin: in vitro evaluation on hela, a2780-adr and h69-ar Molecular Pharmaceutics
Targeted tumor therapy by rubia tinctorum l.: analytical character ization of hydroxyan thraquinones and investigation of their selective Cancer Cell International
Cathelicidins positively regulate pancreatic β-cell functions The FASEB Journal （江南大学发表）
Macrophage polarization and the effect of microrna-155 administered in water-in-oil-in-water multiple emulsion formulations Journal of Clinical & Cellular Immunology
Oloxamer bioadhesive hydrogel for buccal drug delivery: cytotoxicity and trans-epithelial permeability evaluations using tr146 human buccal International Journal of Pharmaceutics
Incubator proof miniaturized holomonitor to in situ monitor cancer cells exposed to green tea polyphenol and preosteoblast cells adhering J. Biomed. Opt
Reversal of chemoresistance in ovarian cancer by co-delivery of mdr inhibitors and paclitaxel using a targeted liposomal platform The FASEB Journal
Induction of morphological changes in death-induced cancer cells monitored by holographic microscopy Journal of Structural Biology
2016
Shielding of the geomagnetic field alters actin assembly and inhibits cell motility in human neuroblastoma cells Scientific Reports (中科院生物物理所发表）
Pseudomonas aeruginosa n-3-oxo-dodecanoyl-homoserine lactone elicits changes in cell volume, morphology, and aqp9 characteristics in Frontiers in Cellular and Infection Microbiology
The curcumin analog c-150, influencing nf-κb, upr and akt/notch pathways has potent anticancer activity in vitro and in vivo PLOS ONE
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