吡喃伊多糖

GlycanPac AXH-1 多糖分析柱Thermo Scientific™ GlycanPac™ AXH-1 LC 色谱柱适用于生物分子中存在的不带电荷和带电荷多糖分子的定性分析、定量分析以及结构表征。 此款高性能、以硅胶为填料的高效液相色谱 (HPLC) 柱可以同时通过电荷、尺寸和极性对多糖分子进行分离。 可以通过 LC-荧光和 LC-质谱 (MS) 方法对标记或天然的生物学上重要的多糖分子进行高分离度和高通量分析，并具备独特的选择性。描述GlycanPac AXH-1 LC 色谱柱利用创新的混合模式表面化学特性，以及弱阴离子交换 (WAX) 和亲水作用色谱（HILIC）保留机理，以实现最佳选择性和分离度。 WAX 功能可以为带有负电荷的多糖提供保留性和选择性，同时 HILIC 模式可以根据其极性和分子尺寸，分离带相同电荷的多糖。 因此，GlycanPac AXH-1 LC 色谱柱可以提供无与伦比的多糖分离能力。 基于电荷、分子尺寸和极性的独特多糖选择性对天然和标记的多糖均具有出色的分离度对于高分离度多糖特性表征和基于电荷的简易多糖定量分析均具有重要作用兼容荧光和 MS 检测方法高色谱效率和出色的色谱柱稳定性GlycanPac AXH-1 LC 色谱柱以高纯度和球型硅胶基质为填料，具有 1.9 和 3μm 两种规格，分别适合 UHPLC 和标准 HPLC 应用。 GlycanPac AXH-1 LC 色谱柱可以满足您应用中的不同分离度、通量和仪器要求的需求。 GlycanPac AXH-1 多糖分析柱订货信息：分析柱GlycanPac AXH-1, 3 μm, Analytical (4.6x150mm)082468GlycanPac AXH-1, 3 μm, Analytical (3.0x150mm)082469GlycanPac AXH-1, 3 μm, Analytical (2.1x150mm)082470GlycanPac AXH-1, 1.9 μm, Analytical (2.1x250mm)082521GlycanPac AXH-1, 1.9 μm, Analytical (2.1x150mm)082472GlycanPac AXH-1, 1.9 μm, Analytical (2.1x100mm)082473GlycanPac AXH-1, 1.9 μm, Analytical (1.0 × 150 mm, with nanoViper)164863保护柱GlycanPac AXH-1, 3 μm, Guard (4.6x10 mm) 2ea (Use Holder V-2)082474GlycanPac AXH-1, 3 μm, Guard (3.0x10 mm) 2ea (Use Holder V-2)082475GlycanPac AXH-1, 3 μm, Guard (2.1x10 mm) 2ea (Use Holder V-2)082476Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188GlycanPac AXH-1 Columns for the High-Resolution Separation and Analysis of GlycansThe Thermo Scientific™ GlycanPac™ AXH-1 column is a high-performance, silica-based HPLC column for simultaneous separation of glycans by charge, size, and polarity. It is designed for high-resolution and high-throughput analysis with unique selectivity for biologically important glycans, either labeled or native, by LC-fluorescence and LC-mass spectrometry (MS) methods.The GlycanPac AXH-1 column can be used for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules (e.g., proteins).Features:Unique glycan selectivity based on charge, size, and polarityExcellent resolution for both native and labeled glycansUseful for both high-resolution glycan profile characterization and easy quantification of glycans based on chargeCompatible with fluorescence and MS detection methodsHigh chromatographic efficiency and excellent column stabilityThe GlycanPac AXH-1 column, based on innovative mixed-mode surface chemistry, combines both weak anion-exchange (WAX) and hydrophilic interaction liquid chromatography (HILIC) retention mechanisms for optimal selectivity and resolution power. The WAX functionality provides retention and selectivity for negatively charged glycans, while the HILIC mode facilitates the separation of glycans of the same charge according to their polarity and size. As a result, the GlycanPac AXH-1 column provides unparalleled capabilities for glycan separations. Additionally, this column offers charge-based separations for various glycans without discrimination of size and polarity, making it a suitable tool for accurate quantification of glycans which cannot be achieved with other HPLC columns. The GlycanPac AXH-1 column is designed and tested for LC-fluorescence detection and LC-MS applications using volatile aqueous buffers (e.g., ammonium acetate or ammonium formate) and acetonitrile.GlycanPac AXH-1 columns are based on high-purity and spherical silica substrates in both 1.9 and 3 μm sizes for UHPLC and standard HPLC applications, respectively. GlycanPac AXH-1 columns are available in various dimensions for applications with different resolutions, throughput, and instrument requirements.The GlycanPac AXH-1 column can be used for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules. The glycan elution profile consists of a series of peaks grouped into several "clusters" in which the neutral glycans elute first, followed by mono-sialylated, di-sialylated, tri-sialylaled species.Analytes in each cluster, retained by anion exchange interactions, represents the glycans of the same charge. Within each cluster the glycans having the same charge are further separated according to their sizes and polarity by HILIC interaction.Similar separation of N-glycans using a 3μm packing material with Fluorescence detection.Separation of N-glycans using the GlycanPac AXH-1, 1.9μm packing with LC/MS detection.Accurate and easy quantitative analysis of relative amounts of glycans of each charge state is possible by using a modified gradient.Separation of human IgG using the GlycanPac AXH-1, 1.9μm column.Analytical ColumnsGlycanPac AXH-1, 3 μm, Analytical (4.6x150mm)082468GlycanPac AXH-1, 3 μm, Analytical (3.0x150mm)082469GlycanPac AXH-1, 3 μm, Analytical (2.1x150mm)082470GlycanPac AXH-1, 1.9 μm, Analytical (2.1x250mm)082521GlycanPac AXH-1, 1.9 μm, Analytical (2.1x150mm)082472GlycanPac AXH-1, 1.9 μm, Analytical (2.1x100mm)082473GlycanPac AXH-1, 1.9 μm, Analytical (1.0 × 150 mm, with nanoViper)164863Guard ColumnsGlycanPac AXH-1, 3 μm, Guard (4.6x10 mm) 2ea (Use Holder V-2)082474GlycanPac AXH-1, 3 μm, Guard (3.0x10 mm) 2ea (Use Holder V-2)082475GlycanPac AXH-1, 3 μm, Guard (2.1x10 mm) 2ea (Use Holder V-2)082476Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188DataSheetGlycanPac AXH-1 ColumnBrochuresThermo Scientific BioLC ColumnsManualsThermo Scientific GlycanPac AXH-1 Columns Product ManualGLYCANPAC AXH-1 COLUMN Quick StartPostersNovel Glycan Column Technology for the LC-MS Analysis of Labeled and Native N-Glycans Released from Proteins and Antibodies

纳微科技开发了UniPS系列专用于多糖分析的高效液相色谱柱，采用三种交联度（5%，8%和10%）的单分散均一粒径阳离子交换微球填料，提供氢型、钠型、钙型的色谱柱和客户定制色谱柱，兼具离子配位交换和分子体积排阻色谱的多重作用机制。多糖分析柱规格参数一览表 *更多规格型号或定制，请联系业务代表订货信息 *更多规格型号或定制，请联系业务代表

TSKgel SuperMultiporePW 色谱柱是分离大多数水溶性合成聚合物及多糖类物质的首选色谱柱。该系列色谱柱装填有小粒径、细孔多分散型填料的半微型色谱柱（6.0 mm ID X 15 cm），可实现超高速、高效分离，节省溶剂。此类色谱柱的校正曲线线性优异、没有拐点，避免了色谱图中出现不正常的凹凸现象。根据样品分子量和分子量分布情况的不同，该系列色谱柱共有3中不同规格可供选择。TSKgel SuperMultiporePW色谱柱适用于分析中性低聚物，在一半的分析时间内即可达到与常规的30cm色谱柱相同的分离性能。药品和保健食品中使用的肝素和硫酸软骨素为酸性多糖，可使用TSKgel SuperMultipore PW色谱柱，以硝酸钠等洗脱液进行快速分离。水溶性聚合物及多糖类分析用TSKgel色谱柱的选择方法硫酸软骨素的分离产品规格产品货号产品名称粒径色谱柱尺寸0022789SuperMultiporePW-N4 um6.0 mm IDX15 cm0022790SuperMultiporePW-M5 um6.0 mm IDX15 cm0022791SuperMultiporePW-H8 um6.0 mm IDX15 cm0022793guardcolumn SuperMP(PW)-N--4.6 mm IDX3.5 cm0022794guardcolumn SuperMP(PW)-M--4.6 mm IDX3.5 cm0022795guardcolumn SuperMP(PW)-H--4.6 mm IDX3.5 cm