透析液中阳离子检测

智能文字提取功能测试中

透析液中钠离子、钾离子、镁离子、钙离子分析报告青岛睿谱分析仪器有限公司摘要：本方法采用RPIC-2017色谱仪、CS12A色谱柱、WLK-10C抑制器，通过等度淋洗在25分钟内即完成钠离子、钾离子、镁离子、钙离子洗脱。将实际样品连续进样6次，钠离子标准溶液r值0.9991，钾离子标准溶液r值0.9992，镁离子标准溶液r值0.9993，钙离子标准溶液r值0.9992。色谱条件：离子色谱仪：RPIC-2017型离子色谱仪色谱柱：CS12A(4×250mm)+CG12A(4×50mm) 淋洗液：MSA15mM（RPEG-1-A产生）流速：1.0mL/min抑制器：WLK-10C 4mm免维护抑制器检测器：动态量程电导检测器进样体积：5L温度：30℃色谱曲线及叠加图：离子Std1Std2Std3Std4Std5Na+5ppm10ppm20ppm30ppm50ppmK+0.25ppm0.5ppm1ppm1.5ppm2.5ppmMg2+0.02ppm0.04ppm0.08ppm0.12ppm0.2ppmCa2+0.2ppm0.4ppm0.8ppm1.2ppm2ppm外标曲线：离子外标曲线方程相关系数rNa+Y=3.6945X+1.32450.9991K+Y=2.4231X-0.16520.9992Mg2+Y=3.3015X-0.01460.9993Ca2+Y=4.0648X-0.38480.9992实际样品：将红盖样品稀释2000倍，白盖样品稀释100倍进样。通过外标法得到其浓度，根据稀释倍数计算得到原溶液浓度。白盖样品叠加谱图红盖样品叠加谱图样品检测结果：样品离子测得浓度ppm原液结果ppm原液结果mM平均值mM红盖2000Na+36.2874229.673228.783204.2135.4272473.843152.4135.5572742.303164.0835.9073457.443195.1936.7975279.363274.4436.0773806.113210.36K+1.152362.9360.4459.471.122293.5758.661.122300.1258.831.132309.3259.061.162368.8760.591.132316.9059.26Mg2+0.19395.1216.2516.210.19393.8916.200.20407.4016.760.19387.3415.930.18374.0415.390.20406.7816.73Ca2+0.831700.1842.4242.090.821682.9941.990.811651.6841.210.821684.4242.030.831700.7942.440.831701.4042.45白盖100Na+30.363247.57141.26138.0129.233127.05136.0230.533266.63142.0929.623168.73137.8328.573057.06132.9729.633169.96137.88K+0.7277.221.981.930.7075.321.930.7377.931.990.7075.291.930.6772.171.850.7175.561.93Mg2+0.1111.930.490.450.1010.620.440.1011.210.460.1011.030.450.099.700.400.1010.510.43Ca2+0.5053.491.331.260.4749.971.250.4952.341.310.4750.111.250.4548.131.200.4649.741.24结语：使用RPIC-2017型离子色谱仪测定透析液，白盖样品钠离子含量为138.01mmol/L，钾离子含量1.93mmol/L，镁离子含量为0.45mmol/L，钙离子含量1.26mmol/L。红盖样品钠离子含量为3204.21mmol/L，钾离子含量59.47mmol/L，镁离子含量为16.21mmol/L，钙离子含量42.09mmol/L。对于浓差较大的多种离子检测，我们推荐使用高容量色谱柱及抑制电导检测。因为其有以下优势：①色谱柱寿命长，②对低浓度组分的测定更灵敏。