饲料中粗纤维检测方案(纤维测定仪)

智能文字提取功能测试中

APPLICATION NOTEF&F-F-003-2019/A1 IVELPSCIENTIFICACF DETERMINATION IN FEED Crude Fiber Determination in Feed(Weende method) Reference： ISO 6865：2000 Animal feeding stuffs-Determination of crude fibre content AOAC 978.10 Fiber (Crude) in Animal Feed and Pet Food REG CE 152/2009 Tested with VELP Scientifica FIWE Advance Fiber AutoExtractor (Code F30500500)and COEX Cold Extractor (Code F30520204) Introduction Fiber is found in the cell walls of plants， and functions sort of like a skeleton for the plants to help maintain their shapeand structure. Plant fiber is mainly consisted of cell wall which is comprised of substances such as cellulose，hemicellulose， ligning and pectine. Fiber is nutritionally important because it represents the organic portion of feeds and foods that is the most difficult todigest. Forage quality is a direct reflection of essential nutrient content and availability to the consuming animals and generallythere is a close relationship between the crude fiber content and the nutrition value of the feed： the higher the crude fiberis， the lower the nutritional value is. Fiber is thus an important parameter to be determined in feed. The performance of VELP FIWE Advance Fiber AutoExtractor was evaluated by participating in the proficiency testingprogram organized by BIPEA. Samples were analyzed using FIWE Advance Fiber analyzer and the obtained resultswere compared with the BIPEA tolerance range. Determination of Crude Fiber content in different feed samplesThe CF determination can be summed up in 5 steps： *performed with COEX During the ACID DIGESTION (BOILING & FILTERING) the feed sample is boiled in sulfuric acid 1，25 % for theextraction of sugar and starch. During WASHING the residues into the crucibles are washed with water to remove acid residues and neutralize the pH.The second digestion is the ALKALY DIGESTION with 1.25 % sodium or potassium hydroxide which removes proteins，some hemicellulose and lignin. Then the WASHING step is performed again in order to remove the alkali residues intothe crucibles with water and neutralize the pH. The final step is the DEFATTING of the samples where the last washesare performed with acetone. BIPEA Samples Common wheatID：12-1113-0188CF assigned value： 2.6 %Tolerance range： 1.8-3.4%Feed for pigletID： 12-1513-0207CF assigned value： 3.2 %Tolerance range： 2.4-4.0 %Production cornID： 15-0513-0073CF assigned value： 2.0 %Tolerance range： 1.2-2.8 % Chemicals and Equipment required Analytical balance， 4 decimals Glass Crucibles P2， 6pcs (A00000140) Sulfuric acid (H2SO4) 1.25%-0.255±0.005N. 12.5 g，98% concentrated to 1000 ml with distilled water. Potassium hydroxide (KOH) 1.25%-0.223±0.005 N， free from carbonate. 12.5 g to 1000 ml with distilled water. ● n-octanol as antifoaming agent Acetone， technical grade Crucibles Preparation Connect the optional VELP barcode reader (Barcode scanner with USB socket Code： A00000364 or Wireless barcode scannerCode： A00000365) to the FIWE Advance. Select Analysis/Details， scan the crucible， weigh 1 g of the sample portion intoeach crucible (Msample) and transfer the Msample value from the balance to FIWE Advance. This operation is repeatedsequentially for the remaining positions. Digestion and filtration with FIWE AdvanceSelect“Analysis”and then method“CF-Crude fiber (Weende method)" including the following parameters： e Crucibles porosity P2·Digestion time： 30 minutes· Preheat： Yes· Washing： 3 x 50 ml of distilled water· Enzyme： No·KOH： 150 ml· H2SO4： 150ml·Digestion time： 30 minutes· N-octanol： Yes·Washing： 3x50 ml of distilled water Lower the lever and position the heating shield. Press START to begin the process. At the end of analysis remove the crucibles from the unit， place them in COEX unit fordefatting (25 ml acetone for 3 times) and dry the crucibles (130°℃±2°C). Leave to cool in the desiccator. In Result menuselect the crucibles batch ID analyzed， press calculate， scan the crucibles with barcode reader and weigh to the nearest0，0001 g (Mdry). Ignite the crucible with the residue in a muffle (525±15℃) for at least 3 h or until carbon-free. Leave tocool in a desiccator. In Result menu select the crucibles batch ID analyzed， press calculate， scan the crucibles withbarcode reader and weigh to the nearest 0，0001 g (Mash).Results on feed samples % Crude Fiber= Mdry-Mash * 100Msample Mary= sample weight after drying Mash = sample weight after ashing Msample = sample weight Sample Msample (g) Mdry (g) Mash (g) CF% Common wheat 0.9958 31.2625 31.2364 2.6210 1.0009 31.3139 31.2874 2.6476 1.0112 31.0186 30.9923 2.6009 Average ±SD% 2.62±0.02 RSD%* 0.9 Feed for piglet 1.1584 31.0613 31.0227 3.3322 0.9958 31.5771 31.5437 3.3541 1.0856 31.5345 31.4978 3.3806 Average ±SD% 3.36±0.02 RSD% 0.7 Production corn 1.0555 30.9845 30.9634 1.9991 1.0795 31.3327 31.3108 2.0287 1.0079 30.9953 30.9748 2.0339 Average ±SD% 2.02±0.02 RSD%* 0.9 ( *RSD%=(Standard Deviation *100) / Average ) Conclusion The obtained results are reliable and in accordance with the expected values， with a low relative standard deviation(RSD ≤1%)， that means high repeatability of the results. The use of an extraction apparatus purposely devised for this method as FIWE Advance unit， makes very easy thestandardization of analytical conditions. The FIWE Series is suitable for Crude Fiber (CF) determination and fiberfractions. Benefits of FIWE Advance are： 6 positions simultaneously and unsupervised Easy to use： 7"touch screen operation with preset method and favorite methods setting Automatic heating and dispensing of reagents avoiding any possible contact with chemicals and fumes State-of-the-art safety features controlling all the steps of the analysis Precision and accuracy： high reproducibility of the results：±1% relative or better ● Results in accordance with official procedures ● Connection to VELP Ermes cloud platform to monitor and control the instrument and to access to your database. In order to avoid losses of fiber， it’s important to remember that crucibles life is around 20-30 analysis， because the fritted filter could bedamaged from basic and acid solutions. Hence it’s sugges ted to change them after 20-30 analysis.      纤维存在于植物的细胞壁中，其功能类似于植物的骨架，以帮助它们的形状和结构。 植物纤维主要由细胞壁组成纤维素、半纤维素、木质素和果胶等物质组成。 纤维在营养上很重要，因为它代表了饲料和食品中最难食用的有机部分消化。 牧草品质直接反映了食用动物的必需养分含量和可利用性，一般情况下，粗纤维含量与食用动物之间存在着密切的关系饲料的营养价值：粗纤维越高，营养价值越低。 因此，纤维是进料中需要确定的重要参数。 VELP FIWE Advance全自动纤维素测定仪的性能通过参加BIPEA组织的熟练程度测试程序，对纤维素分析仪进行了评估。 用FIWE Advance全自动纤维素测定仪对样品进行了分析，并将所得结果与BIPEA公差范围进行比较。实验样品：Common wheat  ID: 12-1113-0188 CF assigned value: 2.6 % Tolerance range: 1.8 – 3.4 %Feed for piglet   ID: 12-1513-0207   CF assigned value: 3.2 % Tolerance range: 2.4 – 4.0 % Production corn  ID: 15-0513-0073  CF assigned value: 2.0 % Tolerance range: 1.2 – 2.8 %检测仪器：FIWE Advance全自动纤维素测定仪   意大利VELP公司检测结果： 得到的结果是可靠的，符合期望值，相对标准偏差较低(RSD≤1%)，这意味着结果的重复性很高。该仪器的使用使分析条件的标准化非常容易。 本系列产品适用于粗纤维(CF)测定和纤维含量分析。  仪器优势特点：►6个位置同时和无监督的自动过程►节省宝贵的时间:只需2分钟的操作时间►使用方便:7触摸屏操作，预设方法和喜爱的方法设置►自动加热和分配试剂，避免任何可能接触化学品和烟雾►先进的安全特性，控制分析的所有步骤►精度高，重复性好：±1%相对或更好。►结果符合标准程序►连接到VELPErmes云平台，监测和控制仪器和访问您的数据库。