deng2010huan 2009/01/14
EN14362 LMBG 82.02-Z 4氨基偶氮苯 LMBG 82.02-9我这里有一份LMBG 82.02的SOP,应该对你有用 1. Purpose This operation procedure is provided to detect the use of certain azo dye, which can release 4-aminoazobenzene during reductive cleavage of its azo bond, from textile or leather commodity articles. 2. Reference Amtilche Sammlung von Untersuchungsverfahren nach §35 LMBG Untersuchung von Bedarfsgegenstaenden (nachweis der Verwendung von Azofarbstoffen, die 4- Aminoazobenzol freisetzen koennen) 3. Chemicals 4-AAB is a known carcinogen to human being, thus its handling should under special caution. Unless specified, all chemicals should be in analytical grade. 3.1 NaOH (AR) 3.2 Sodium dithionite solution, k1) = 200 mg/mL, freshly prepared 3.3 2% NaOH (solution) 3.4 n-Hexane (AR) 3.5 Methanol (AR) 3.6 Chlorobenzene (AR) 3.7 tert-Butylmethylether (AR) 3.8 NaCl (AR) 3.9 4-Aminoazobenzene (highest grade) 3.10 Internal standard for GC - IS1: Naphthaline-d8, CAS# 1146-65-2 - IS2: 2,4,5-Trichloroaniline, CAS# 636-30-6 - IS4: Anthracene-d10, CAS# 1719-06-08 3.11 Standard solution - 4-AAB calibrate solution, k1) = 15.0 μg 4-AAB/mL in an appropriate solvent - internal standard solution, k1) = 10.0 μg IS/mL in an appropriate solvent - 4-AAB control solution, k1) = 500 μg 4-AAB/mL Methanol; 1) k = measured concentration 4. Apparatus 4.1 Analytical balance accurate to 0.1 mg 4.2 20 to 50 mL reaction flask (heat-resistant) with teflon-sealer 4.3 100 mL round bottom flask (NS 29/32) with reflux condenser 4.4 Extraction apparatus (refer to §35 LMBG B82.02-4, figure 1) 4.5 Heater with thermostat 4.6 Centrifuge 4.7 Vacuum rotary evaporator 4.8 Pipette 10 mL, 5 mL, 2 mL, and 1 mL. 4.9 Ultrasonic bath with heat regulator 4.10 Dispenser 4.11 Mechanical shaker providing horizontal shaking motion, IKA HS260 or equivalent 4.12 GC/MS, Agilent 6890GC, with agilent 5975 mass spectrometer or equivalent
穿越时空 2009/09/29
楼主不回答,算应助了.
deng2010huan
第2楼2009/01/14
EN14362
LMBG 82.02-Z 4氨基偶氮苯
LMBG 82.02-9我这里有一份LMBG 82.02的SOP,应该对你有用
1. Purpose
This operation procedure is provided to detect the use of certain azo dye, which can release 4-aminoazobenzene during reductive cleavage of its azo bond, from textile or leather commodity articles.
2. Reference
Amtilche Sammlung von Untersuchungsverfahren nach §35 LMBG
Untersuchung von Bedarfsgegenstaenden (nachweis der Verwendung von Azofarbstoffen, die 4- Aminoazobenzol freisetzen koennen)
3. Chemicals
4-AAB is a known carcinogen to human being, thus its handling should under special caution. Unless specified, all chemicals should be in analytical grade.
3.1 NaOH (AR)
3.2 Sodium dithionite solution, k1) = 200 mg/mL, freshly prepared
3.3 2% NaOH (solution)
3.4 n-Hexane (AR)
3.5 Methanol (AR)
3.6 Chlorobenzene (AR)
3.7 tert-Butylmethylether (AR)
3.8 NaCl (AR)
3.9 4-Aminoazobenzene (highest grade)
3.10 Internal standard for GC
- IS1: Naphthaline-d8, CAS# 1146-65-2
- IS2: 2,4,5-Trichloroaniline, CAS# 636-30-6
- IS4: Anthracene-d10, CAS# 1719-06-08
3.11 Standard solution
- 4-AAB calibrate solution, k1) = 15.0 μg 4-AAB/mL in an appropriate solvent
- internal standard solution, k1) = 10.0 μg IS/mL in an appropriate solvent
- 4-AAB control solution, k1) = 500 μg 4-AAB/mL Methanol; 1) k = measured concentration
4. Apparatus
4.1 Analytical balance accurate to 0.1 mg
4.2 20 to 50 mL reaction flask (heat-resistant) with teflon-sealer
4.3 100 mL round bottom flask (NS 29/32) with reflux condenser
4.4 Extraction apparatus (refer to §35 LMBG B82.02-4, figure 1)
4.5 Heater with thermostat
4.6 Centrifuge
4.7 Vacuum rotary evaporator
4.8 Pipette 10 mL, 5 mL, 2 mL, and 1 mL.
4.9 Ultrasonic bath with heat regulator
4.10 Dispenser
4.11 Mechanical shaker providing horizontal shaking motion, IKA HS260 or equivalent
4.12 GC/MS, Agilent 6890GC, with agilent 5975 mass spectrometer or equivalent
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