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【每周一问】在200-300nm处光谱扫描遇到的问题

07jlfs

2008/07/25

紫外可见分光光度计（UV）

数据如下:
"WL","Abs","%Trans"
"200.0","-0.301030","200.0000"
"201.0","-0.301030","200.0000"
"202.0","-0.301030","200.0000"
"203.0","-0.301030","200.0000"
"204.0","-0.301030","200.0000"
"205.0","-0.301030","200.0000"
"206.0","3.000000","-1.#IND"
"207.0","3.000000","-1.#IND"
"208.0","3.000000","-1.#IND"
"209.0","3.000000","-1.#IND"
"210.0","-0.301030","200.0000"
"211.0","-0.301030","200.0000"
"212.0","-0.301030","200.0000"
"213.0","-0.301030","200.0000"
"214.0","-0.301030","200.0000"
"215.0","3.000000","-1.#IND"
"216.0","3.000000","-1.#IND"
"217.0","3.000000","-1.#IND"
"218.0","3.000000","-1.#IND"
"219.0","3.000000","-1.#IND"
"220.0","3.000000","-1.#IND"
"221.0","3.000000","-1.#IND"
"222.0","3.000000","-1.#IND"
"223.0","3.000000","-1.#IND"
"224.0","3.000000","-1.#IND"
"225.0","3.000000","-1.#IND"
"226.0","3.000000","-1.#IND"
"227.0","3.000000","-1.#IND"
"228.0","3.000000","-1.#IND"
"229.0","3.000000","-1.#IND"
"230.0","-0.301030","200.0000"
"231.0","3.000000","-1.#IND"
"232.0","3.000000","-1.#IND"
"233.0","3.000000","-1.#IND"
"234.0","3.000000","-1.#IND"
"235.0","3.000000","-1.#IND"
"236.0","3.000000","-1.#IND"
"237.0","3.000000","-1.#IND"
"238.0","3.000000","-1.#IND"
"239.0","3.000000","-1.#IND"
"240.0","3.000000","-1.#IND"
"241.0","3.000000","-1.#IND"
"242.0","3.000000","-1.#IND"
"243.0","3.000000","-1.#IND"
"244.0","3.000000","-1.#IND"
"245.0","3.000000","-1.#IND"
"246.0","3.000000","-1.#IND"
"247.0","3.000000","-1.#IND"
"248.0","3.000000","-1.#IND"
"249.0","3.000000","-1.#IND"
"250.0","3.000000","-1.#IND"
"251.0","3.000000","-1.#IND"
"252.0","3.000000","-1.#IND"
"253.0","3.000000","-1.#IND"
"254.0","3.000000","-1.#IND"
"255.0","3.000000","-1.#IND"
"256.0","3.000000","-1.#IND"
"257.0","3.000000","-1.#IND"
"258.0","-0.301030","200.0000"
"259.0","-0.301030","200.0000"
"260.0","3.000000","-1.#IND"
"261.0","3.000000","-1.#IND"
"262.0","3.000000","-1.#IND"
"263.0","3.000000","-1.#IND"
"264.0","3.000000","-1.#IND"
"265.0","3.000000","-1.#IND"
"266.0","3.000000","-1.#IND"
"267.0","3.000000","-1.#IND"
"268.0","3.000000","-1.#IND"
"269.0","3.000000","-1.#IND"
"270.0","3.000000","-1.#IND"
"271.0","3.000000","-1.#IND"
"272.0","3.000000","-1.#IND"
"273.0","3.000000","-1.#IND"
"274.0","3.000000","-1.#IND"
"275.0","3.000000","-1.#IND"
"276.0","3.000000","-1.#IND"
"277.0","3.000000","-1.#IND"
"278.0","3.000000","-1.#IND"
"279.0","-0.301030","200.0000"
"280.0","-0.301030","200.0000"
"281.0","-0.301030","200.0000"
"282.0","-0.301030","200.0000"
"283.0","-0.301030","200.0000"
"284.0","-0.301030","200.0000"
"285.0","-0.301030","200.0000"
"286.0","-0.169751","147.8261"
"287.0","-0.101027","126.1905"
"288.0","-0.061937","115.3285"
"289.0","-0.040474","109.7674"
"290.0","-0.030700","107.3248"
"291.0","-0.021325","105.0328"
"292.0","-0.012326","102.8788"
"293.0","-0.008544","101.9868"
"294.0","-0.004325","101.0008"
"295.0","-0.000843","100.1943"
"296.0","-0.000445","100.1025"
"297.0","0.001447","99.6674"
"298.0","0.002986","99.3148"
"299.0","0.003078","99.2938"
"300.0","0.004446","98.9815"
这是什么情况...请教各位大侠,,是不是基线没调整好??
但是若在单220nm波长处测Abs,好像数据满正常的,,,

相关话题

初学者&九点虎

第1楼2008/07/26

能把吸收光谱图传上来吗
这样看数据有点乱，谢谢

0

07jlfs

第2楼2008/07/26

不好意思,,,那个文件是tst格式,只能用记事本打开...
图上因该是这样的,,有很多段高的平台,,然后一下子下到最低,,又突然是一段很高的A=3.000的平台...

0

savedown

第3楼2008/07/27

估计是光源出问题了

0

初学者&九点虎

第4楼2008/07/27

重新做一次，看能不能重现，最好把图传上来
07jlfs 发表: 不好意思,,,那个文件是tst格式,只能用记事本打开...
图上因该是这样的,,有很多段高的平台,,然后一下子下到最低,,又突然是一段很高的A=3.000的平台...

0

savedown

第5楼2008/07/27

0

savedown

第6楼2008/07/27

光源在紫外段功率弱，吸收小的几个地方是光源的锐线，接近可见部分光源功率开始变得正常，可以得到平稳响应。

0

07jlfs

第7楼2008/07/28

感谢这位大侠,,,,另外我用单点测吸光度,貌似得到了正确的数据,,但是数据有个问题,就是数据好像很不稳定,,从大到小,,,再从小到大...从0.02几变到0.008,是不是也佐证了上述问题??谢谢啦.
savedown 发表:光源在紫外段功率弱，吸收小的几个地方是光源的锐线，接近可见部分光源功率开始变得正常，可以得到平稳响应。

0

savedown

第8楼2008/07/28

估计是光源不稳定的抖动造成的。换一个好的紫外光源试试看。

0

初学者&九点虎

第9楼2008/07/28

我觉得仪器最大测量吸光度范围3，感觉超过了测量范围，是不是溢出了，稀释一倍看看什么情况，如果还这样，可能就光源问题
savedown 发表:

0

07jlfs

第10楼2008/07/30

感谢各位大侠的热心回答,
不好意思各位,出现这张图的原因已被我找到,,
原来比色皿是玻璃的不是石英...汗..
吸收太强,超出范围,这种情况下基线根本无法调整,导致了混乱的数据
这台仪器因为是新进的,氘灯我想应该是好的.
还是谢谢大家啦.

0

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