作 者:傅军李焕丹高晓霞梁从庆潘志青 FU Jun, LI Huandan, GAO Xiaoxia, LIANG Congqing, PAN Zhiqing (Guangdong College of Pharmacy, Guangzhou 510006 ) 摘 要:目的 建立保和口服液中连翘苷的含量测定方法.方法 采用HPLC法,色谱柱为Diamonsil C18柱,流动相:乙腈-水(29:71),流速为1.0 mL·min-1,检测波长277 nm,柱温为室温.结果 连翘苷在0.068~0.340μg范围内线性关系良好,相关系数r=0.9996;平均回收率为99.7%(RSD=2.1%).结论 该方法简便,快速,准确,重复性好,可作为保和口服液的质控指标之一. Objective To establish a method for the determination offorsythin in Baohe Oral Liquid by HPLC. Methods The determination was carried out on a Diamonsil C18 column, with mobile phase of acetonitrile-water(29:71) at room temperature. The flow rate was 1.0mL·min^-1 and the detective wavelength at 277 nm. Results The calibration curves was linear in the range of 0.068-0.340μg (r=0.9996) . The average recovery rate of tested sample was 99.7 % (RSD=2.1%). Conclusion The method was specific, accurate and precise. It can be used for the quality control of Baohe Oral Liquid. 关 键 词:保和口服液高效[url=https://insevent.instrument.com.cn/t/5p]液相色谱法[/url] 连翘苷含量测定Baohe Oral LiquidForsythinHPLCContent determination
qq250083771(qq250083771)发表:作 者:傅军李焕丹高晓霞梁从庆潘志青 FU Jun, LI Huandan, GAO Xiaoxia, LIANG Congqing, PAN Zhiqing (Guangdong College of Pharmacy, Guangzhou 510006 ) 摘 要:目的 建立保和口服液中连翘苷的含量测定方法.方法 采用HPLC法,色谱柱为Diamonsil C18柱,流动相:乙腈-水(29:71),流速为1.0 mL·min-1,检测波长277 nm,柱温为室温.结果 连翘苷在0.068~0.340μg范围内线性关系良好,相关系数r=0.9996;平均回收率为99.7%(RSD=2.1%).结论 该方法简便,快速,准确,重复性好,可作为保和口服液的质控指标之一. Objective To establish a method for the determination offorsythin in Baohe Oral Liquid by HPLC. Methods The determination was carried out on a Diamonsil C18 column, with mobile phase of acetonitrile-water(29:71) at room temperature. The flow rate was 1.0mL·min^-1 and the detective wavelength at 277 nm. Results The calibration curves was linear in the range of 0.068-0.340μg (r=0.9996) . The average recovery rate of tested sample was 99.7 % (RSD=2.1%). Conclusion The method was specific, accurate and precise. It can be used for the quality control of Baohe Oral Liquid. 关 键 词:保和口服液高效[url=https://insevent.instrument.com.cn/t/5p]液相色谱法[/url] 连翘苷含量测定Baohe Oral LiquidForsythinHPLCContent determination