【Author】 Lu Yingnian,Wu Kefeng,Liang Nianci(Guangdong Medical College,Guangdong Key Lab for Research and Development of Natural Drugs,Zhanjiang,Guangdong,China 524023)
【Abstract】 Objective To establish a HPLC method for content determination of apigenin from Pteris semipinnata L..Methods The separation was performed on a Diamonsil C18 column,the mobile phase was the mixture of methanol(B)and water(A)with a linear gradient elution at a flow rate of 0.2 mL/min.The ultraviolet wavelength was 283 nm,and the column temperature was maintained at 35℃.Results The calibration curve showed good linearity in the range of 0.076-0.76 μg(r2=0.998 6),the average recovery rate was 98.1%... 更多还原 【关键词】 高效液相色谱法; 半边旗; 黄酮; 芹菜素; 【Key words】 HPLC; Pteris semipinnata L.; flavonoid; apigenin;