Mobile Phase B | 10 mM Ammonium Acetate (pH=4.0) | ||||||||||||||
Mobile Phase A | Acetonitrile (LC-MS Grade) | ||||||||||||||
Column | Waters Acquity HIILIC,2.1*100 mm, 1.7um | ||||||||||||||
Column Temperature | 35 | ||||||||||||||
Flow ratio | 0.3 mL/min | ||||||||||||||
Gradient |
|
Transition | Fragmentor | CE | Dwell Time (ms) |
85.0-68.0 (Quantitative) | 50 | 20 | 400 |
85.0-43.0 (Qualitative) | 50 | 10 | 400 |
ESI Model | Positive |
Gas Flow | 13 mL/min |
Pressure | 50 Psi |
Gas Temperature | 350 |
Capillary Voltage | 2000 V |
Resolution | Unit (both) |
EMV | 200 |
Divert Valve | From 0.5 min to 3 min to MS |
1. 1 ppb DCD Standard