色谱条件：
色谱柱: SeQ ant® ZIC® Column: SeQuant-HILIC (5 μm, 200Å) PEEK 250x4.6 mm 1.50458.0001
检测器: Fluorescence detection (Excitation=350nm, Emission=395nm)
流速: 0.7 mL/min
流动相 (v/v): A: 10 mM Ammonium formate and 10 mM formic acid in Milli-Q® water (100%)
B: acetonitrile and Milli-Q® water with Itot 8 mM Ammonium formate (80:20)
温度：室温
梯度：
Time(min) | Solution A (%) | Solution B (%) | Elution |
0-24.0 | 18 | 82 | Isocratic |
24.1-35.0 | 30 | 70 | Isocratic |
35.1-50.0 | 35 | 65 | Isocratic |
50.1-70.0 | 18 | 82 | Equilibration |
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