凝胶色谱的问题

应助达人

查到的sephadex G15的信息，不知道是不是和你的一样，这G15没有说明书吗？

Properties
Related Categories
Gel Filtration Chromatography, Gel Filtration Media, Molecular Biology, Protein Chromatography, Proteomics More...
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature
autoclavable In wet form (pH 7.0) at 120?C for 30 min
packaging
pack of 100 g
mfr. no.
GE Healthcare, 17-0020-01
parameter
flow rate (The beads behave as rigid spheres obeying Darcy′s Law.)
matrix
cross-linked dextran
particle size
40-120 μm (dry)

60-180 μm (wet)
working range
2 - 13
Show Fewer Properties
Description
Analysis Note
www.gelifesciences.com.
Application
Sephadex? is a gel filtration medium prepared by crosslinking Dextran with epichlorohydrin. Different types of Sephadex? differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex? G-15 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex? G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations at where high flow rates and Low operating pressures are required.
Features and Benefits
? Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
? Suitable for buffer exchange and cleanup of biological samples, for example peptides, small Proteins, and oligosaccharides larger than 1 500 molecular weight
? Efficient removal of contaminants such as salts, dyes, and radioactive labels
General description
Sephadex? G-15 is well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules > 1500 molecular weight.
Legal Information
Sephadex is a registered trademark of GE Healthcare