询问溶菌酶的液相 流动相的条件

你的整体柱是什么基质的？给你几个溶菌酶的色谱分析条件参考一下吧。
Mobile Phase: A = 95% Water: 5% ACN with 0.1% TFA B = 5% Water: 95% ACN with 0.085% TFA Flow Rate: a: Analytical 1 mL / min b: Narrow Bore 0.2 mL / min Gradient: 10-60% B in 30 min.

Mobile Phase: A = 5:95 ACN:Water with 0.10% TFA (v/v%) B = 95:5 ACN:Water with 0.085% TFA (v/v%) Flow Rate: 1.0 mL/min Gradient: 15-53% in 20 min., posttime 12 min.

Mobile Phase: A = 0.01 M sodium phosphate, pH 6.0 B = 0.01 M sodium phosphate in 0.5 M NaCl, pH 6.0 Flow Rate: 1.0 mL/min Gradient: 0 to 100% B in 30 min