null检测 | 结合荧光显微镜及原子力显微镜对细胞骨架形态的研究-布鲁克纳米表面仪器部(Bruker Nano Surfaces)

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Fluorescence microscopy has become an indispensable tool in cell biology because it allows specifi c proteins to be visualized. Atomic force microscopy (AFM) is also becoming extensively used in the life sciences, but its development has largely followed an independent path and is used for somewhat different, but often complementary, purposes. Both methods can be applied to hydrated biological samples. Fluorescence microscopy is routinely used to image and track specifi cally labeled biological molecules in situ, illuminating a wide variety of cellular processes. In contrast, AFM is used for high spatial resolution imaging but also introduces the possibility of mechanically probing and manipulating samples without the need of complicated sample preparation. In this note, we briefl y describe the application of an AFM mounted on an inverted optical microscope to the characterization of the morphology of axons from cultured chick neurons using specifi c fl uorescent immunostaining and topographic imaging with AFM.

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上一篇台阶仪在三维形貌表征领域的应用

下一篇骨胶原纤维的大范围高分辨AFM形貌图分析

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布鲁克纳米表面仪器部(Bruker Nano Surfaces)

白金会员丨第20年

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