So you benefit from
1
So you benefit from Up to 0.5 Å increase in resolution on replacing traditional holey carbon supports with UltrAuFoil®.¹
2
The highest resolution data from your initial movie frames before radiation damage accumulates, due to reduced beam-induced motion.² ³
3
Reduced image distortion because of lowered static and semi-mobile charge accumulation as gold is highly conductive at liquid nitrogen temperatures.²
4
Samples protected from damage due to accumulating positive charge by neutralization with secondary electrons generated by irradiating adjacent gold.²
5
Biomolecules retaining native structure and not being put under mechanical strain from foil crinkling.
6
Improved particle distribution as the gold foil
is even flatter than carbon.²
7
Easier grid surveying due to the high contrast of the gold foil.²
References
1
Danev, R. et al. Routine sub-2.5 Å cryo-EM structure determination of GPCRs. Nat. Commun. 12: 4333 (2021).
2
Russo, C. R. & Passmore, L. A. Ultrastable gold substrates: Properties of a support for high-resolution electron cryomicroscopy of biological specimens. J. Struct. Biol. 193: 33-44 (2016).
3
Russo, C. R. & Passmore, L. A. Ultrastable gold substrates for electron cryomicroscopy. Science 346: 1377-1380 (2014).
4
Amil, S. M. et al & Fernandez-Leiro, R. The cryo-EM Structure of Thermotoga maritima β-Galactosidase: Quaternary Structure Guides Protein Engineering. ACS Chem. Biol. 15: 179-188 (2020).
Choosing the right grid
mesh and foil geometry
UltrAuFoil® specifications
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