其他null检测 | 采用化疗可咯和多模光学成像方法研究光子激发诱导线粒体损伤-北京欧兰科技发展有限公司

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应用领域	医疗/卫生
检测样本	其他
检测项目
参考标准	暂无

采用LaVision公司独有的，快门宽度最小可达50皮秒的增强型CCD相机，通过化疗可咯和多模光学成像方法对光子激发诱导线粒体损伤机理进行了研究。

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We recently reported that a targeted, brightly fluorescent gallium corrole (HerGa) is highly effective for
breast tumor detection and treatment. Unlike structurally similar porphryins, HerGa exhibits tumor-targeted toxicity
without the need for photoexcitation. We have now examined whether photoexcitation further modulates HerGa
toxicity, using multimode optical imaging of live cells, including two-photon excited fluorescence, differential interference
contrast (DIC), spectral, and lifetime imaging. Using two-photon excited fluorescence imaging, we
observed that light at specific wavelengths augments the HerGa-mediated mitochondrial membrane potential
disruption of breast cancer cells in situ. In addition, DIC, spectral, and fluorescence lifetime imaging enabled
us to both validate cell damage by HerGa photoexcitation and investigate HerGa internalization, thus allowing
optimization of light dose and timing. Our demonstration of HerGa phototoxicity opens the way for development
of new methods of cancer intervention using tumor-targeted corroles.

上一篇在一个双稳湍流涡旋火焰中，对间歇性动态的时间-频率定位

下一篇微钻孔和数字图像相关技术（DIC）测量材料微尺度残余应力

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北京欧兰科技发展有限公司

金牌会员丨第17年

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