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HPLC Conditions ba058 Column : CAPCELL PAK Ca MGII S5: 4.6 mm i.d. x250 mm Mobile phase : Trifluoroacetic acid was added to a solution (water / methanol =10 / 90) at 0.1 vol% Flow rate :1.0mL/min Temperature :40℃ Detection : UV 215 nm Inj. vol. :5pL Pretreatment : The standard compounds were dissoled in methanol at 1 pg/mL. Soymilk wasdiluted 5-fold with methanol, and then shaked for 30 seconds. After the solutionwas centifuged (1500 pm) for 30 seconds, the upper layer was filtered with a0.2-pm flter and introduced to HPLC ※ 1 pg/mL=1 ppm
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