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HPLC Conditions ba060 Column :CAPCELL PAK Ca MGII S5 :4.6 mm i.d.x 250 mm Mobile phase : CH:0H Flow rate : 1.0 mL/min Temperature : 40℃ Detection : RI Inj. vol. : 20 pL Pretreatment : The standard compounds were dissolved in chloroform at 10 mg/ mL, and diluted to 1 pg/mL with methanol. Soymilk was diluted5-fold with methanol, and then shaked for 30 seconds. After thesolution was centrifuged (1500 rpm) for 30 seconds, the upperlayer was filtered with a 0.2-pm filter and introduced to HPLC※1 pg/mL=1 ppm
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