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HITACHI1nspire the NextTechnical ReportAS/MSD-005 Liquid Chromatographhttp：//www.hitachi-hitec-science.com2015 Hitachi High-Tech Science Corporation Analysis of Reserpine by Mass Detector Chromaster 5610 MS Detector is a mass detector with new concept， designed for LCusers， and it is different from conventional mass spectrometers. This time， reserpine wasquantitatively analyzed as a model sample. Reserpine is a component analyzed as astandard sample by LC-MS. The calibration curve generated based on the SIMchromatograms showed a good linearity. 5610 MS Detector LC-MS Analysis of Reserpine ■ Analytical Conditions Table 2 Conditions for HPLC Setting Table 1 Conditions for MS Detector Setting lonization method ESI lonization mode Positive lonization voltage 2500V Measurementmode SIM (m/z 609.3) Column HITACHI LaChrom C18 (5 um)4.6 mml.D. x 150 mm Mobile phase CH3CN/H2O/HCOOH=30/70/0.1 Flow rate 1.0 mL/min (250：1 sprit) Column temperature 25℃ Detection wavelength UV 270 nm Injection vol. 20pL LC-MS Analysis Reserpine was dissolved in methanol. Figure 1 shows the results of reserpine (1 mg/L) analysed by UV and massdetectors. The [M+H]t ion peak at m/z 609.3 was detected in the mass spectrum. In a conventional HPLC system， theflow channel is split for the connection with a mass detector. In the SIM chromatogram of reserpine， the sensitivity wasfound to be equivalent to that obtained by UV detection. Figure 2 shows the calibration curve of reserpinebased on the SIM chromatograms. Agoodlinearity was obtained over the concentrationrange of 0.01-100 mg/L. Figure 1 Chromatogram of Reserpine Figure 2Calibration Curve Based on SIM Chromatograms
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日立科学仪器（北京）有限公司为您提供《利血平中含量检测方案(液质联用仪)》，该方案主要用于化药制剂中限度检查检测，参考标准《暂无》，《利血平中含量检测方案(液质联用仪)》用到的仪器有null。
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