糖醇的半微量高效液相色谱分析

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与传统分离相比，半微量HPLC分析在减少分离时间和通过减少流动相的消耗来降低成本方面提供了显著的优势。然而，由于在未优化的流动路径中的峰扩散，设计用于常规分离的HPLC系统通常不能提供足够好的峰形状或分离。当使用半微柱时，建议使用优化的RHPLC系统。该分析报告比较了使用常规折射率检测器和针对半微量HPLC优化的检测器对糖醇的测量。Application Note 2/2Analysis of Sugar Alcohols by Semi-micro HPLC Analysis of Sugar Alcohols by Semi-micro HPLC I ntroduction Semi -micro HPLC analysis o f fers a significant advantage compared with conventional separations in reducing separation t ime and cut t ing cost by reducing the consumption of m obile phase. However， HPLC systems designed for conventiona l separations often do not provide good enoug h peak shape or separation due to peak diffusion in t he non-optimized f low path. When using a semi -micro column an optimi z ed RHPLC system is recommended . This anal y sis report compares the measurement of sugar alcohols using a conventional refractive i n dex detector wi t h a detector optimized for semi-micro HPLC . Keyword： HPLC， Sugar Alcohols， Semi -micro， Amide column， RI [Equipmen t ]ICondi t ions Pump： PU-4185Column：Inertsi l Amide (2.1 mml .D. x250 mmL ，3 um)Pump option： Degasser unit Eluent：Water/Acetonit r ile (30/70)Autosampler： AS-4150Flow rate：0.2 mL/min Column oven： CO-4060Column temp.：30°℃Detector： RI-4035Inject i on vol u me：1pL Standard Sample：Sugar alcohols mixture (5 mg/mL each) F i g. 1 shows t he chromatograms of sugar alcohols measured using a refractive index detector for conventional analysis (Rl-4030) or for semi-micro analysis (RI-4035). Using the Rl -4035 for sem i -m icro anal y sis can obviously provide improved peak shape and resolution. Figure 1. Chromat o gram of Sugar al co hol samples 1： G l yceri n e， 2： Eryt h rit o l ，3： Xylit o l ， 4： Solbitol， 5： I n ositol P/N Description 7003-J014A PU-4185 RHPLC Semi-micro Pump (Base Unit) 7006-H003A DG-4000-04 4-channel Degasser Unit， for Analytical 7064-J002A AS-4150 RHPLC Autosampler 7021-J002A CO-4060 Column Oven 7031-J002A RI-4035 Refractive Index Detector， for Semi-micro LC 7058-J011A BS-4000-1 Bottle stand 6688-H564A LC-Net CG cable (3x) 7059-J012A ChromNAV Ver.2 Chromatography Data System 7001-H403A RHPLC/UHPLC Start Up Kit for LC-4000 7001-H405A Maintenance tool kit Inertsil Amide (2.1 mml.D. x250 mmL，3 pm)