第1楼2005/03/21
从文献看血铅的测定目前好像最多是石墨炉原子吸收,全血中多元素的测定一般是:
取40ul血样用1ml抗凝剂(通常是Triton X-100)混匀直接用原子吸收石墨炉测定。
,必须进行背景校正,最好用平台管,有条件的情况下使用10%的氧气加入载气中,用以烧尽石墨管(或平台)上沉积的碳。没有平台管,不加氧气也行。
钨舟电热原子化也是可行的方法,国内博辉仪器公司生产的BH2100型钨舟原子吸收测铅仪在国内市场上不少。仪器使用S-H法背景校正,他对测铅正好是很合适的。所以能得到很好的数据。两天前,中央电视台报道的北京儿研所对全国儿童铅中毒的调查报告,所采用的大量数据都是统一用这个型号的仪器测定的。我参加了这个项目的验收。
较之用原子吸收,这是一个专用仪器。仪器简单体积小,操作也方便,分析者易于掌握,耗能低(仅300瓦),耗材(钨舟)也较便宜。一个医院如果仅测铅,买原子吸收就不值了。
随便找了几篇文献,较老了。摘要不全,给了一点预处理方法。有两篇是使用钨舟或钨丝电热原子化器的,有一篇是使用S-H背景校正的。供参考。
1.
1.1 ml 0.8 mol l-1 HNO3 in 0.1% Triton X-100 was added to 0.1 ml blood,left for 15 min and centrifuged. The deproteinized supernatant wasanalysed by AAS
2.
Residues after digestion with HClO4-HNO3 were diluted with 0.1 mol l-1HCl. 0.2 ml from the solution was injected into an FI manifold to mixwith 1% nitroso-R salt in 40 mmol l-1 NaOH and with 0.5% NaBH
3.
Blood specimens diluted ten-fold with 0.1% v/v Triton X-100 wereanalysed using 500 .mu.g l-1-2% m/v citric acid-0.1 mol l-1 HNO3 as thechemical modifier. Increased concentrations were found in patient
4.
A simple, low-cost prototype instrument was developed for measurement of Pb in blood. It includes a tungsten filament atomizer and Smith-Hieftjebackground correction.
5.
A dedicated, low-cost, portable instrument was developed for themeasurement of Pb in blood. It includes a tungsten coil atomizer(powered from a car battery) and measures non-atomic absorption
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