无名
第3楼2008/12/25
For some plastic and rubber products, especially those under the conditions described extraction with toluene largely resolved, is a purification of the extract by silica-Adsorptionschromatograhie required.
This will be a clean-up column with Hahn cut (approximately 220 x 15 mm) with glass wool, 4 grams of silica gel and 1 cm sodium sulfate filled.
It silica gel has previously by the addition of 10% water to disable (the silica glass bulb is the appropriate quantity of water and then 1 h at the Rotary evaporation at room temperature for 760 Torr and homogenized. The gel can then be sealed in glass flasks to be stored at room temperature ).
The conditioning of the column is packed with 10 ml of petroleum.
Then the Toluolextraktaliquot in a rotary evaporator to about 1 ml and confined to the column. The Spitz flask with about 20 ml Eluent off, which is also on the clean-up column is convicted. The elution carried out with 50 ml of petroleum. The hived Petrolethereluat with 1 ml of toluene and moved on TurboVap with nitrogen at approximately 1 ml used restricts. Then, with toluene at a defined volume up and then the extract by GC-MS analysis.
piery2006
第6楼2009/05/25
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