老多_小多
第3楼2010/04/01
纳升色谱没用过,感觉就是LC的改装版色谱,使用的人不多
http://bbs.instrument.com.cn/shtml/20100210/2397205/
是针对纳升级、毛细管、窄径分离而设计的,目的在于获得最高效色谱分辨率、灵敏度及重现性。 直接纳升流速在常规纳升流纳升级液相色谱速分离技术的基础上又有了重大改进。用户将看到改进后的峰容量和峰形,每次分离后检测得到的组分数量增加。
纳升级液相色谱可高效分离各种多肽和蛋白质,与质谱联用后是蛋白组学研究的最佳工具,纳升液相色谱采用微流控制系统,可在20 nL/min 的流量水平下实现精确控制和梯度洗脱;微流控技术也极大地提高了质谱检测的灵敏度,可对更多的低丰度多肽进行分离和鉴定。
此外,纳升级液相色谱还可拓展应用至代谢靶分子、致病蛋白分子的鉴定,在纳升级稳定流量下质谱检测器可获得很好的灵敏度、分辨率和重现性。
老多_小多
第4楼2010/04/01
老多_小多
第5楼2010/04/01
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老多_小多
第6楼2010/04/01
熊猫宝宝
第8楼2010/04/02
说实在这个了解的不多
不过从网上了解一些信息:
Chorus 220 solvent delivery system
Capillary and Nano $$lc
Preparation/Collection of MALDI Targets
Multidimensional Chromatography
Front-end separation for MS proteomics
Isocratic or gradient high pressure pumping system for precise, pulse-free nanoliter fluid delivery
High performance pressure monitored zirconium pistons Direct solvent delivery without flow splitting
Micro version gradient flow range 1ul-70ul,Nano version gradient flow rate 200nl-10ul
Independent solvent channel outlets for low dead volume and rapid gradient equilibrium
Ultra compact design saves valuable benchspace Complete $$lc-MS Front-End together with any CTC PAL sampler loader
Easy to use graphical software package controls gradient formation and monitors flow rate, pressure and system status
Unique Design
The Chorus 220 solvent delivery system achieves precise, pulseless fluid delivery at flow rates as low as 200nl/min. required in today's nanoscale and capillary $$lc. It's unique design incorporating two high pressure syringe pumps connected to a proprietary piston switching valve* delivers accurate gradients that are ideally suited for high sensitivity UV, Fluoresence or electrospray mass spectrometry using capillary column. Both motor-driven and software controlled syringe pumps maintain constant, pulse-free solvent delivery. High pressure mixing and short tubing paths reduces gradient delay and achieves low overall volume.
Splitless Nano Gradient Delivery
The Chorus 220 system provides true nanoliter solvent delivery without flow splitting often used in conventional low flow $$lc pumps. This approach avoids unintentional changes in flow rate due to split ratio fluctuations caused by e.g. increased column resistance or viscosity changes during gradient formation. Furthermore splitless operation results in a real reduction of solvent consumption without recycling costs.
Software control
The Chorus 220 system is operated by a dedicated Windows XP solvent delivery software. Intuitive graphical method editing, flow rate and pressure diagnostics, time programmable valve switching features makes it easy to setup, run and monitor the whole chromatographic process. In manual mode important parameters can easily be varied during system start-up or method development. Third party devices like detectors, autosamplers or column switching valves are straightforward combined by various digital outputs.
Complete CTC Analytics $$lc-MS Front-End
Despite of it's high performance the Chorus 220 measures just 225mm in width. This compact size makes it an ideal companion of any $$lc-MS system. All Chorus systems can easy be placed underneath of one of CTC's PAL sample loaders. The short tubing distance between sample injection valve and Chorus pump results in an optimized ultra low delay volume.