【序号】： 1 【作者】： Praveen Sethupathy, Molly Megraw Artemis G Hatzigeorgiou 【题名】：A guide through present computational approaches for the identification of mammalian microRNA targets 【期刊】：Nature Methods 【年、卷、期、起止页码】： Nature Methods 3, 881 - 886 (2006) 【全文链接】： http://www.nature.com/nmeth/journal/v3/n11/full/nmeth954.html abstract:
Computational microRNA (miRNA) target prediction is a field in flux. Here we present a guide through five widely used mammalian target prediction programs. We include an analysis of the performance of these individual programs and of various combinations of these programs. For this analysis we compiled several benchmark data sets of experimentally supported miRNA–target gene interactions. Based on the results, we provide a discussion on the status of target prediction and also suggest a stepwise approach toward predicting and selecting miRNA targets for experimental testing.
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