寻找DNA磷谱

High-Resolution NMR of DNA and Drug–DNA Interactions
Spectroscopic Methods and Analyses
NMR, Mass Spectrometry, and Metalloprotein Techniques
May 1993
pps. 87-114
ISBN: 0-89603-215-9
Series: Methods in Molecular Biology
Volume #: 17


Jill Barber
Helen F. Cross
John A. Parkinson

Abstract:
The advantage of NMR over most other spectroscopic techniques lies in the ability to gain structural and dynamic information at atomic resolution. Every nucleus with spin gives rise to a signal that is characterized by a number of parameters (chemical shift, J-couplings, relaxation data, and NOE connectivities) that can be used to obtain quite detailed structural information about the molecule under study. They can also be used to determine kinetic properties, for example, the interconversion rates of different conformations of a molecule and the exchange rate of free with bound ligand on a macromolecule. NMR has been widely used to study both static and dynamic aspects of DNA structure and drug–DNA interactions.
Several atomic nuclei are available for the study of DNA by NMR. 1H is the most common, but 31P NMR is especially useful for studying the effects of ligand binding on the phosphate groups of DNA.