Electron Microscopy Principles and Techniques for Biologists Second Edition

mingbright

2006/04/04

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透射电镜（TEM）

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Electron Microscopy

Principles and Techniques for Biologists
Second Edition

John J. Bozzola, M.S., Ph.D.
Center for Electron Microscopy
Southern Illinois University
Carbondale, Illinois

Lonnie D. Russell, M.S., Ph.D.
Laboratory of Structural Biology
School of Medicine
Southern Illinois University
Carbondale, Illinois

Brief Contents

Chapter 1
The Past, Present, and Future of Electron Microscopy

2

Chapter 2
Specimen Preparation for Transmission Electron Microscopy

16

Chapter 3
Specimen Preparation for Scanning Electron Microscopy

48

Chapter 4
Ultramicrotomy

72

Chapter 5
Specimen Staining and Contrast Methods for Transmission Electron Microscopy

120

Chapter 6
The Transmission Electron Microscope

148

Chapter 7
The Scanning Electron Microscope

202

Chapter 8
Production of the Electron Micrograph

240

Chapter 9
Immunocytochemistry

262

Chapter 10
Enzyme Cytochemistry

282

Chapter 11
Autoradiography/Radioautography

292

Chapter 12
Miscellaneous Localization and Enhancement Techniques

310

Chapter 13
Quantitative Electron Microscopy

320

Chapter 14
Freeze Fracture Replication

342

Chapter 15
Analytical Electron Microscopy

368

Chapter 16
Intermediate and High Voltage Microscopy

396

Chapter 17
Tracers

406

Chapter 18
Image Processing and Image Analysis by Computer

414

Chapter 19
Interpretation of Micrographs

442

Chapter 20
Survey of Biological Ultrastructure

476

Chapter 21
Safety in the Electron Microscope Laboratory

616

Appendix A
Review Questions and Problems

641