RP-HPLC Determination and Pharmacokinetic Comparison of Cinnamic Acid in Rat Plasma After Administration of Di-Gu-Pi Decoction and Pure Cinnamic Acid
LI Kang and BI Kai-shun 1．Department ofPharmacy，Shenzhen Hospital，Beijing University，Shenzhen 518036，P．R．China； 2．College of Pharmacy，Shenyang Pharmaceutical University，Shenyang 1 10016，P．R．China
A sensitive。simple．and accurate method was developed for the determination and pharmacokinetic comparison of cinnamic acid in rat plasma after the administration of a Traditional Chinese Medicinal preparation．Di—Gu—Pi de—coction．and pure cinnamic acid using RP．HPLC．Di．Gu．Pi was extracted with 5％ aqueous sodium bicarbonate。which was followed by purification with ion exchange column chromatography．The plasma samples taken from rats were deproteinized with methano1．The reversed—phase(HPI C)system with a Diamonsil Cl8 column and methanolacetonitrile—water(8：32：60，volume ratio)(adjusted to pH：3．0 with glacial acetic acid)as the mobile phase was employed for the separation of cinnamic acid in the plasma samples．The detection was set at 272 nm and 3一(p-fluorophenyl ．2-propenoic acid was chosen as the internal standard．The calibration curve was linear in a range from 0．10to 25．0 g／mL (R =0．9988，n=9)．The precision was 3．42％一10．10％ ；the between—day precision was 2．84％ 一8．91％ ：the accuracy was 一1．51％ 一1．26％ ：the mean recovery was 99．9％ ．The method was found to be sensitive，simple．accurate and appropriate for the detemfination of cinnamic acid．
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