有对AB的时间延迟碎裂了解的比较透彻的么

Time Delayed Fragmentation(TDF) working process:
1. Precursor ion selected in Q1
2. Ions are trapped in Q2(no fragmentation)
3. Ions are accelerated into the trap and fragment through collisionally activated dissociation
4. During the "cool"time,fragments below a user-specified mass are not trapped
5. After the "cool"time,all ions are collectd and scanned out of the trap
6. As ions are being scanned out of the trap,incoming ions are stored in the Q0 region(Q0 trapping)

I wish it would help you a lot.:)

清明节到了，高人都放假了请耐心等待

第三步 离子是怎么碎的？碰撞气是从哪里来的？是在Trap里碎的？尤其是第四步，我记得一些资料上说，cool time是将容易再次碎裂的离子排出离子阱，而留下的仅是母离子的真正子离子，而非孙离子，不懂，求指点

The TDF Mode is similar to Enhance Product Ion Scan,which perform MS/MS scan and Q3 becomes a filter.Fragment the ions in trap by setting Entry barrie between Q2&Q3 (TDF CE...Analyst advanced MS setting),no frag in Q2,Q2 is only used for trap,Q3 is used for frag/trap/scan.

When you set the Q3 Fill Mass in Analyst,during the "cool"time,fragments below the setting value are not trapped.After the "cool"time,all ions are collectd and scanned out of the trap

The grand-daughter ions is produced by MS3 mode.

这有两篇比较早的文献, 可供参考.
不知道如何upload PDF, 只好让大家去下吧.

http://medchem.rutgers.edu/AnalMedChem511/pdf_files/RB_pdf/LIT%20and%20QQQ.pdf
http://onlinelibrary.wiley.com/doi/10.1002/rcm.1061/pdf

记得在Training时, 说TDF主要用在做多肽,也就没有在细问了.