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【求助】baseline problem

液质联用(LCMS)

  • Sorry I couldn't type in Chinese here. I'm working on detection of multiple drugs(8) simutaneously by LC/MS/MS, the baselines for them are not identical. What's the possible reasons and how to optimize? Can I go ahead to do quantification with their very own baseline? Thanks a lot! BTW, how to 结贴? Not only the instrument but also 论坛 is brand new for me.
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  • isomer

    第1楼2007/03/27

    结帖的话
    你点击发的帖子右上角的“维护”进去,就可以看到了



    其他的请高手帮你回答吧

    不过现在凌晨12点半了,可能活动人不是很多。

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  • happyjyl

    第2楼2007/03/27

    给楼主翻译一下:

    楼主在用LC/MS/MS同时检测8个药.这8个药的基线不一致.可能的原因是什么? 如何优化?可以继续做定量检测吗?

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  • 瓢虫

    第3楼2007/03/27

    不同的化合物基线噪音不一样!
    刚优化完化合物质谱条件建议冲洗柱子和仪器1-2天再开始做实验。这样基线会降很多。
    串联质谱很灵敏,所以要注意防止样品污染,样品处理过程要处处想着防止污染的问题。最重要的是如果条件许可的话,样品的称量和样品预处理室要分开。我们以前没分开,三天两头有污染。

    shelby 发表:Sorry I couldn't type in Chinese here. I'm working on detection of multiple drugs(8) simutaneously by LC/MS/MS, the baselines for them are not identical. What's the possible reasons and how to optimize? Can I go ahead to do quantification with their very own baseline? Thanks a lot! BTW, how to 结贴? Not only the instrument but also 论坛 is brand new for me.

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  • shelby

    第5楼2007/03/28

    Thanks for your translation!

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