新问题之二:更换色谱柱后做SIM扫描无可确定的峰?解答:SIM扫描通常用于分组分析和改变各时间段的扫描离子。柱子改变通常导致峰保留时间的改变。如果某一特定化合物峰的保留时间改变了,因为该化合物的特征离子在该时间段未设置,这将导致该化合物峰不出现。在这种情况下,应再进行一次全扫描,以重置SIM的扫描特征离子以进行SIM检测。你也可通过同时进行SIM/Scan 来获得这些数据。
weifengtwo 发表:新问题之二:After replacing column for SIM analysis why are no peaks identified? 更换柱子之后,SIM模式下扫描检测不到任何组分?
解答:SIM analysis typically performs grouping and changes the ions captured in each time band. Changing the column usually causes the peak retention times to change. If the retention time for a particular compound's peak changes the peak will not appear if the ions required to capture that compound's peak are not set. In this situation, perform another scan measurement, reconfigure the SIM data acquisition conditions based on this data, and then perform the SIM measurement. You can also obtain the data by performing synchronous SIM/Scan data acquisition.