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简介：
Principle of the assay The kit assay Rat LDL level in the sample, use Purified Rat LDL antibody to coat microtiter plate wells, make solid-phase antibody, then add LDL to wells, Combined LDL antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat LDL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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