方案摘要
方案下载应用领域 | 生物产业 |
检测样本 | 其他 |
检测项目 | |
参考标准 | / |
It is generally understood that chiral compounds have different bioactivities depending upon the absolute configuration of each compound. Some familiar examples include glutamic acid and thalidomide. Lglutamic acid demonstrates the “Umami” taste*1, while D-glutamic acid has a bitter taste, similarly, the R form of thalidomide is a sedative, but the S form has teratogenic activity. Thus, the separation and study of chiral compounds is critical for many reasons. The functionalities of chiral compounds have been studied for the development of advanced molecules for many applications. The study of chiral compounds has spread to several fields such as natural products, pharmaceuticals and other functional molecules, and it can be pointed out that among those studies, the structural analysis of chiral compounds is a very important topic. X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR) and Electronic Circular Dichroism (ECD) using UV/Vis light are employed as primary methods for the structural analysis of chiral compounds. In this paper, the measurement of chiral compounds by Vibrational Circular Dichroism (VCD) using infrared light will be outlined. VCD is a method to measure the difference of absorbance intensity between left-hand and right-hand circularly polarized light as shown in Figure 1. It is an advantage of VCD that this method can be applied to almost all organic compounds in the same way as infrared (IR) spectroscopy. In addition, by comparing the measurement results with calculated results by ab-initio molecular orbital calculations, the absolute configuration of the sample can be determined. However, since the peak intensity of VCD spectra are 1,000 – 10,000 times weaker than that of standard IR spectra, spectroscopic instruments with high sensitivity and stability with very small baseline fluctuations are required. The FVS-6000 VCD system has a high sensitivity detector, suitable optical
It is generally understood that chiral compounds have different bioactivities depending upon the absolute
configuration of each compound. Some familiar examples include glutamic acid and thalidomide. Lglutamic
acid demonstrates the “Umami” taste*1, while D-glutamic acid has a bitter taste, similarly, the R
form of thalidomide is a sedative, but the S form has teratogenic activity. Thus, the separation and study
of chiral compounds is critical for many reasons.
The functionalities of chiral compounds have been studied for the development of advanced molecules
for many applications. The study of chiral compounds has spread to several fields such as natural
products, pharmaceuticals and other functional molecules, and it can be pointed out that among those
studies, the structural analysis of chiral compounds is a very important topic. X-ray Diffraction (XRD),
Nuclear Magnetic Resonance (NMR) and Electronic Circular Dichroism (ECD) using UV/Vis light are
employed as primary methods for the structural analysis of chiral compounds. In this paper, the
measurement of chiral compounds by Vibrational Circular Dichroism (VCD) using infrared light will be
outlined.
VCD is a method to measure the difference of absorbance intensity between left-hand and right-hand
circularly polarized light as shown in Figure 1. It is an advantage of VCD that this method can be applied
to almost all organic compounds in the same way as infrared (IR) spectroscopy. In addition, by
comparing the measurement results with calculated results by ab-initio molecular orbital calculations, the
absolute configuration of the sample can be determined. However, since the peak intensity of VCD
spectra are 1,000 – 10,000 times weaker than that of standard IR spectra, spectroscopic instruments
with high sensitivity and stability with very small baseline fluctuations are required. The FVS-6000 VCD
system has a high sensitivity detector, suitable optical
HPLC 中的采样频率、响应时间和柱外效应
基于抗体的药物强制降解研究中聚集物和降解产物的SEC监测
用CD光谱法评估VHH抗体的高阶结构、稳定性和相似性
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