特思达

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Abstract. This study investigates the changes in radial micro-texture via Kearn’s f-factors during single cold pilger reductionof a titanium Ti-3-2.5 alloy as a result of strain path changes from tooling modifications. EBSD results confirm that thetexture intensity as well as the radial f-factors can be increased by modifications of pilgering tooling. In addition a switchbetween the secondary prism planes which lie normal to the pilger direction in the starting tube to primary prism planesafter pilgering has been observed.Material and ExperimentalThe tubes investigated were made from Ti alloy 3Al 2.5V, they were manufactured via hot extrusion and then cold pilgered.The cold pilger process configuration is schematically shown in Figure 1. Two strain paths, shown in the ‘Q‘ factor chart inFigure 2, were used in the manufactiure: texture minimised and texture maximised. They were annealed at 750oC beforebeing cold pilgered and for this investigation they were stress relieved at 530oC for 2 hours prior to EBSD examination.Specimens were extracted from two regions either side of the reduced tube as shown in the schematic Figure 2, mountedin conductive Bakelite and polished mechanically for EBSD examination. The final polish was carried out using vibratorypolishes using a mixture of 5:2 colloidal silica, hydrogen peroxide mixture. EBSD examination was conducted using aFEGSEM and OI EBSD Nordlys detector and AZtec Software.

人正常T细胞表达和分泌因子(RANTES/CCL5)检测试剂盒人正常T细胞表达和分泌因子(RANTES/CCL5)检测试剂盒使用说明书本试剂盒仅供研究使用。检测范围： 规格：96T/48T使用目的：本试剂盒用于测定人血清,血浆及相关液体样本中人正常T细胞表达和分泌因子(RANTES/CCL5)含量。实 验 原 理 本试剂盒应用双抗体夹心酶标免疫分析法测定标本中人正常T细胞表达和分泌因子(RANTES/CCL5)水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入人正常T细胞表达和分泌因子(RANTES/CCL5)抗原、生物素化的人正常T细胞表达和分泌因子(RANTES/CCL5)抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的人正常T细胞表达和分泌因子(RANTES/CCL5)呈正相关。 使用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。