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[align=left][font='times new roman'][size=18px]Sil-SMA-MME[/size][/font][font='times new roman'][size=18px]色谱柱分离[/size][/font][font='times new roman'][size=18px]核苷[/size][/font][font='times new roman'][size=18px]/[/size][/font][font='times new roman'][size=18px]核酸碱基类化合物[/size][/font][font='times new roman'][size=18px]及[/size][/font][font='times new roman'][size=18px]苯酚类化合物[/size][/font][/align][font='times new roman'][size=16px]选用亲水性的核苷[/size][/font][font='times new roman'][size=16px]/[/size][/font][font='times new roman'][size=16px]核酸碱基类物质考察了[/size][/font][font='times new roman'][size=16px]Sil-SMA-MME[/size][/font][font='times new roman'][size=16px]色谱柱的分离性能。[/size][/font][font='times new roman'][size=16px]如图[/size][/font][font='times new roman'][size=16px],使用[/size][/font][font='times new roman'][size=16px]乙腈[/size][/font][font='times new roman'][size=16px]-[/size][/font][font='times new roman'][size=16px]水([/size][/font][font='times new roman'][size=16px]9[/size][/font][font='times new roman'][size=16px]4:6[/size][/font][font='times new roman'][size=16px],[/size][/font][font='times new roman'][size=16px]v/v[/size][/font][font='times new roman'][size=16px])[/size][/font][font='times new roman'][size=16px]作为流动相对待测样品进行洗脱,在[/size][/font][font='times new roman'][size=16px]8 [/size][/font][font='times new roman'][size=16px]min[/size][/font][font='times new roman'][size=16px]以内,[/size][/font][font='times new roman'][size=16px]尿苷、尿嘧啶和肌苷三[/size][/font][font='times new roman'][size=16px]种物质可以达到基线分离([/size][/font][font='times new roman'][size=16px]a[/size][/font][font='times new roman'][size=16px])[/size][/font][font='times new roman'][size=16px],[/size][/font][font='times new roman'][size=16px]色谱图峰型良好,[/size][/font][font='times new roman'][size=16px]三[/size][/font][font='times new roman'][size=16px]物质的理论塔板数均大于[/size][/font][font='times new roman'][size=16px]60[/size][/font][font='times new roman'][size=16px],[/size][/font][font='times new roman'][size=16px]000 N/m[/size][/font][font='times new roman'][size=16px]。采用[/size][/font][font='times new roman'][size=16px]SiO[/size][/font][font='times new roman'][sub][size=16px]2[/size][/sub][/font][font='times new roman'][size=16px]-NH[/size][/font][font='times new roman'][sub][size=16px]2[/size][/sub][/font][font='times new roman'][size=16px]色谱柱[/size][/font][font='times new roman'][size=16px]对以上[/size][/font][font='times new roman'][size=16px]三[/size][/font][font='times new roman'][size=16px]种物质进行分离,在相同的流动相条件下,[/size][/font][font='times new roman'][size=16px]SiO[/size][/font][font='times new roman'][sub][size=16px]2[/size][/sub][/font][font='times new roman'][size=16px]-NH[/size][/font][font='times new roman'][sub][size=16px]2[/size][/sub][/font][font='times new roman'][size=16px]色谱柱对于[/size][/font][font='times new roman'][size=16px]核苷[/size][/font][font='times new roman'][size=16px]/[/size][/font][font='times new roman'][size=16px]核酸碱基[/size][/font][font='times new roman'][size=16px]类物质的保留更[/size][/font][font='times new roman'][size=16px]强[/size][/font][font='times new roman'][size=16px],[/size][/font][font='times new roman'][size=16px]15 min[/size][/font][font='times new roman'][size=16px]之[/size][/font][font='times new roman'][size=16px]内只有[/size][/font][font='times new roman'][size=16px]尿嘧啶[/size][/font][font='times new roman'][size=16px]出峰(图[/size][/font][font='times new roman'][size=16px]b[/size][/font][font='times new roman'][size=16px])[/size][/font][font='times new roman'][size=16px]。[/size][/font][font='times new roman'][size=16px]不断调节流动相中[/size][/font][font='times new roman'][size=16px]ACN[/size][/font][font='times new roman'][size=16px]和[/size][/font][font='times new roman'][size=16px]H[/size][/font][font='times new roman'][sub][size=16px]2[/size][/sub][/font][font='times new roman'][size=16px]O[/size][/font][font='times new roman'][size=16px]的比例,[/size][/font][font='times new roman'][size=16px]选用[/size][/font][font='times new roman'][size=16px]流动相[/size][/font][font='times new roman'][size=16px]乙腈[/size][/font][font='times new roman'][size=16px]-[/size][/font][font='times new roman'][size=16px]水([/size][/font][font='times new roman'][size=16px]60:40[/size][/font][font='times new roman'][size=16px],[/size][/font][font='times new roman'][size=16px]v/v[/size][/font][font='times new roman'][size=16px])[/size][/font][font='times new roman'][size=16px]时,[/size][/font][font='times new roman'][size=16px]三种物质中保留时间最长的肌苷与在[/size][/font][font='times new roman'][size=16px]Sil-SMA-MME[/size][/font][font='times new roman'][size=16px]柱上的保留时间基本一致,三种物也可以达到基线分离,[/size][/font][font='times new roman'][size=16px]但尿嘧啶和尿苷的分离度仅[/size][/font][font='times new roman'][size=16px]1[/size][/font][font='times new roman'][size=16px].62[/size][/font][font='times new roman'][size=16px]。[/size][/font][align=center][img=,690,528]https://ng1.17img.cn/bbsfiles/images/2023/08/202308031653067829_7939_3237657_3.png!w690x528.jpg[/img][/align][align=center][img]" style="max-width: 100% max-height: 100% [/img][/align][align=center][font='times new roman'] [/font][font='times new roman']核苷[/font][font='times new roman']/[/font][font='times new roman']核酸碱基类物质在[/font][font='times new roman']Sil-SMA-MME[/font][font='times new roman']色谱柱([/font][font='times new roman']a[/font][font='times new roman'])和[/font][font='times new roman']SiO[/font][font='times new roman'][sub][size=13px]2[/size][/sub][/font][font='times new roman']-NH[/font][font='times new roman'][sub][size=13px]2[/size][/sub][/font][font='times new roman']柱([/font][font='times new roman']b[/font][font='times new roman'],[/font][font='times new roman']c[/font][font='times new roman'])中的分离色谱图[/font][/align][align=center][font='times new roman']色谱条件:图[/font][font='times new roman']a[/font][font='times new roman']和[/font][font='times new roman']b[/font][font='times new roman']流动相为乙腈[/font][font='times new roman']-[/font][font='times new roman']水([/font][font='times new roman']94:6[/font][font='times new roman'],[/font][font='times new roman']v/v[/font][font='times new roman']),图[/font][font='times new roman']c[/font][font='times new roman']流动相为乙腈[/font][font='times new roman']-[/font][font='times new roman']水([/font][font='times new roman']60:40[/font][font='times new roman'],[/font][font='times new roman']v/v[/font][font='times new roman']);检测波长,[/font][font='times new roman']214 nm[/font][font='times new roman'];流速,[/font][font='times new roman']1.0 mL/min[/font][/align][align=center][font='times new roman']分析物:[/font][font='times new roman']1. [/font][font='times new roman']尿嘧啶;[/font][font='times new roman']2. [/font][font='times new roman']尿苷;[/font][font='times new roman']3. [/font][font='times new roman']肌苷[/font][/align][align=center][font='times new roman']Separation chromatograms of nucleosides and nucleic acid bases on the Sil-SMA-MME column (a) and commercial amino column (b, c)[/font][/align][align=center][font='times new roman']Chromatographic conditions: mobile phase, ACN-H[/font][font='times new roman'][sub][size=13px]2[/size][/sub][/font][font='times new roman']O (94:6, v/v) for a and b, ACN-H[/font][font='times new roman'][sub][size=13px]2[/size][/sub][/font][font='times new roman']O (60:40, v/v) for c [/font][font='times new roman'] [/font][font='times new roman']detection wavelength, 214 nm flow rate, 1.0 mL/min[/font][/align][align=center][font='times new roman']Analytes: 1. [/font][font='times new roman']u[/font][font='times new roman']racil 2. [/font][font='times new roman']u[/font][font='times new roman']ridine 3. [/font][font='times new roman']i[/font][font='times new roman']nosine[/font][/align][align=left][font='times new roman'][size=18px] Sil-SMA-MME[/size][/font][font='times new roman'][size=18px]色谱柱分离[/size][/font][font='times new roman'][size=18px]苯酚类化合物[/size][/font][/align][font='times new roman'][size=16px]最后,[/size][/font][font='times new roman'][size=16px]选取[/size][/font][font='times new roman'][size=16px]四种苯酚[/size][/font][font='times new roman'][size=16px]类小分子物质对[/size][/font][font='times new roman'][size=16px]Sil-SMA-MME[/size][/font][font='times new roman'][size=16px]色谱[/size][/font][font='times new roman'][size=16px]柱[/size][/font][font='times new roman'][size=16px]的分离性能进行考察[/size][/font][font='times new roman'][size=16px]。[/size][/font][font='times new roman'][size=16px]为[/size][/font][font='times new roman'][size=16px]对乙酰氨基酚、对甲酚、对氯苯酚和[/size][/font][font='times new roman'][size=16px]4[/size][/font][font='times new roman'][size=16px]-[/size][/font][font='times new roman'][size=16px]叔辛基苯酚[/size][/font][font='times new roman'][size=16px]的色谱[/size][/font][font='times new roman'][size=16px]分离[/size][/font][font='times new roman'][size=16px]图。以流动相乙腈[/size][/font][font='times new roman'][size=16px]-[/size][/font][font='times new roman'][size=16px]水([/size][/font][font='times new roman'][size=16px]40:60[/size][/font][font='times new roman'][size=16px],[/size][/font][font='times new roman'][size=16px]v/v[/size][/font][font='times new roman'][size=16px])进行洗脱,[/size][/font][font='times new roman'][size=16px]四种物质在[/size][/font][font='times new roman'][size=16px]6[/size][/font][font='times new roman'][size=16px] [/size][/font][font='times new roman'][size=16px]min[/size][/font][font='times new roman'][size=16px]之内可以实现基线分离,[/size][/font][font='times new roman'][size=16px]色谱图峰型良好[/size][/font][font='times new roman'][size=16px],四[/size][/font][font='times new roman'][size=16px]种物质的理论塔板数均大于[/size][/font][font='times new roman'][size=16px]5[/size][/font][font='times new roman'][size=16px]5[/size][/font][font='times new roman'][size=16px],[/size][/font][font='times new roman'][size=16px]000 N/[/size][/font][font='times new roman'][size=16px]m[/size][/font][font='times new roman'][size=16px]。采用[/size][/font][font='times new roman'][size=16px]SiO[/size][/font][font='times new roman'][sub][size=16px]2[/size][/sub][/font][font='times new roman'][size=16px]-NH[/size][/font][font='times new roman'][sub][size=16px]2[/size][/sub][/font][font='times new roman'][size=16px]色谱[/size][/font][font='times new roman'][size=16px]柱[/size][/font][font='times new roman'][size=16px]对以上[/size][/font][font='times new roman'][size=16px]四[/size][/font][font='times new roman'][size=16px]种物质进行分离,在相同的流动相条件下,[/size][/font][font='times new roman'][size=16px]SiO[/size][/font][font='times new roman'][sub][size=16px]2[/size][/sub][/font][font='times new roman'][size=16px]-NH[/size][/font][font='times new roman'][sub][size=16px]2[/size][/sub][/font][font='times new roman'][size=16px]色谱[/size][/font][font='times new roman'][size=16px]柱[/size][/font][font='times new roman'][size=16px]对于[/size][/font][font='times new roman'][size=16px]苯酚[/size][/font][font='times new roman'][size=16px]类物质的保留更[/size][/font][font='times new roman'][size=16px]弱[/size][/font][font='times new roman'][size=16px],[/size][/font][font='times new roman'][size=16px]对乙酰氨基酚和对甲酚色谱峰完全重叠,对氯苯酚和[/size][/font][font='times new roman'][size=16px]4[/size][/font][font='times new roman'][size=16px]-[/size][/font][font='times new roman'][size=16px]叔辛基苯酚也无法达到基线分离[/size][/font][font='times new roman'][size=16px]。调节流动相比例,使保留时间最长的[/size][/font][font='times new roman'][size=16px]4[/size][/font][font='times new roman'][size=16px]-[/size][/font][font='times new roman'][size=16px]叔辛基苯酚[/size][/font][font='times new roman'][size=16px]与在[/size][/font][font='times new roman'][size=16px]Sil-SMA-MME[/size][/font][font='times new roman'][size=16px]色谱柱上保留时间基本一致,此时,流动相为[/size][/font][font='times new roman'][size=16px]乙腈[/size][/font][font='times new roman'][size=16px]-[/size][/font][font='times new roman'][size=16px]水([/size][/font][font='times new roman'][size=16px]9[/size][/font][font='times new roman'][size=16px]7:3[/size][/font][font='times new roman'][size=16px],[/size][/font][font='times new roman'][size=16px]v/v[/size][/font][font='times new roman'][size=16px])[/size][/font][font='times new roman'][size=16px],[/size][/font][font='times new roman'][size=16px]四种物质可以达到基线分离,但分离选择性与在[/size][/font][font='times new roman'][size=16px]Sil-SMA-MME[/size][/font][font='times new roman'][size=16px]色谱柱上[/size][/font][font='times new roman'][size=16px]有显著差异。[/size][/font][align=center][img=,689,549]https://ng1.17img.cn/bbsfiles/images/2023/08/202308031653171485_5277_3237657_3.png!w689x549.jpg[/img][/align][align=center][img]" style="max-width: 100% max-height: 100% [/img][/align][align=center][font='times new roman'] [/font][font='times new roman']苯酚类物质在[/font][font='times new roman']Sil-SMA-MME[/font][font='times new roman']色谱柱([/font][font='times new roman']a[/font][font='times new roman'])和[/font][font='times new roman']SiO[/font][font='times new roman'][sub][size=13px]2[/size][/sub][/font][font='times new roman']-NH[/font][font='times new roman'][sub][size=13px]2[/size][/sub][/font][font='times new roman']柱([/font][font='times new roman']b[/font][font='times new roman'],[/font][font='times new roman']c[/font][font='times new roman'])中的分离色谱图[/font][/align][align=center][font='times new roman']色谱条件:图[/font][font='times new roman']a[/font][font='times new roman']和[/font][font='times new roman']b[/font][font='times new roman']流动相为乙腈[/font][font='times new roman']-[/font][font='times new roman']水([/font][font='times new roman']40:60[/font][font='times new roman'],[/font][font='times new roman']v/v[/font][font='times new roman']),图[/font][font='times new roman']c[/font][font='times new roman']流动相为乙腈[/font][font='times new roman']-[/font][font='times new roman']水([/font][font='times new roman']97:3[/font][font='times new roman'],[/font][font='times new roman']v/v[/font][font='times new roman']);检测波长,[/font][font='times new roman']214 nm[/font][font='times new roman'];流速,[/font][font='times new roman']1.0 mL/min[/font][/align][align=center][font='times new roman']分析物:[/font][font='times new roman']1. [/font][font='times new roman']对乙酰氨基酚;[/font][font='times new roman']2. [/font][font='times new roman']对甲酚;[/font][font='times new roman']3.[/font][font='times new roman']对氯苯酚;[/font][font='times new roman']4. 4-[/font][font='times new roman']叔辛基苯酚[/font][/align][align=center][font='times new roman']Separation chromatograms of phenols on the Sil-SMA-MME column (a) and commercial amino column (b, c)[/font][/align][align=center][font='times new roman']Chromatographic conditions: mobile phase, ACN-H[/font][font='times new roman'][sub][size=13px]2[/size][/sub][/font][font='times new roman']O (40:60, v/v) for a and b, ACN-H[/font][font='times new roman'][sub][size=13px]2[/size][/sub][/font][font='times new roman']O (97:3, v/v) for c detection wavelength, 214 nm flow rate, 1.0 mL/min[/font][/align][align=center][font='times new roman']Analytes: 1. acetaminophen 2. [/font][font='times new roman']p[/font][font='times new roman']-cresol 3. [/font][font='times new roman']p[/font][font='times new roman']-chlorophenol 4. 4-[/font][font='times new roman']tert[/font][font='times new roman']-octylphenol[/font][/align][align=left][font='times new roman'][size=20px]小结[/size][/font][/align][font='times new roman'][size=16px]采用苯乙烯和马来酸酐作为单体原材料,通过“点击”反应与自由基聚合反应将苯乙烯[/size][/font][font='times new roman'][size=16px]-[/size][/font][font='times new roman'][size=16px]马来酸酐共聚物接枝在硅胶表面,然后通过亲核开环反应,用[/size][/font][font='times new roman'][size=16px]M[/size][/font][font='times new roman'][size=16px]ME[/size][/font][font='times new roman'][size=16px][/size][/font][font='times new roman'][size=16px]HC[/size][/font][font='times new roman'][size=16px]l[/size][/font][font='times new roman'][size=16px]对共聚物中的马来酸酐基团进行开环修饰,制备了[/size][/font][font='times new roman'][size=16px]Sil-SMA-MME[/size][/font][font='times new roman'][size=16px]色谱固定相材料。采用热重分析和红外光谱分析证明了[/size][/font][font='times new roman'][size=16px]Sil-SMA-MME[/size][/font][font='times new roman'][size=16px]固定相的成功制备。[/size][/font][font='times new roman'][size=16px]Sil-SMA-MME[/size][/font][font='times new roman'][size=16px]色谱柱具有亲水相互作用保留机制。通过小分子物质,对[/size][/font][font='times new roman'][size=16px]Sil-SMA-MME[/size][/font][font='times new roman'][size=16px]色谱柱的分离性能进行考察,结果显示该色谱柱具有良好的分离选择性和较高的理论塔板数,有望进一步用于磷脂的分离分析。[/size][/font]
主体内容(操作步骤):开机:打开计数器后面的开关,显示Instrument Initialising 启动需数秒Instrument Ready选择“Set up”键,这部可在快速吸光度读数时省略设对照“Set reference”将石英杯插入孔中,按箭头指示方向使光线方向在前到后轴线上。测样品:Instrument up 设置如果键入错误数字,set按“C”重新开始set up and enter按set up选择并输入与你样品相关的参数,要退出“set up”按任一计数键,要重新开始,按“set up”石英杯杯内径(mm)按“select”从0.5、1、5、10中选择^Prrneter(打印)按“select”选择ON或OFF 按`Enter'^Sample number(样品数)键入需要数目按 “enter”样品数,每次测量样品时都会自动累加。^Date(日期)键入日期,按“enter”(每日调节)^Moth(月)选择正确月,按“enter”^year (年)键入年,按“enter”backarouad compersation 320nm(背景补偿)320nm按“select”选择Yes or No^Dilute(稀释)键入稀释度,计算浓度,范围1.00-9999.9Factor(因子)按“select”选RNA dsDNA ssDNA对合成寡核苷酸选择ssDNABases碱基,键入A C G T V数通过核酸碱基对组成,计算分子量,以每毫升分子数显示结果按“enter”循环碱基,范围0-1000Oligo length (寡核苷酸长度)以碱基单位键入样本寡核苷酸长度以pmol/ul显示结果,按“enter”范围0-100Molecular weightMW(分子量)按“select”在计算值从A 、C 、G 、T、 V或使用者输入分子量值间选者,按“enter”Ratio 键入A260/A280吸光度比值(你希望样本达到的)按“enter”Concentration(浓度)键入希望浓度(pmol/ul)按“enter”Protein(蛋白)键入系数^Molarity(名称克分子浓度)键入盐浓度测对照:按 Set ref,听到“嘟”一声后将含对照石英杯插入样品槽中,听到“嘟”一声后,取出参照,屏幕上显示吸光度测样品步骤同对照,按“Sample”后同对照。
阿维菌素和伊维菌素的化学结构中有没有酸碱基团? 溶液的酸碱性?谢谢~~~~~