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Hitachi High-Technologies Corporation Analysis of NO, in Water Sample U-5100 INTRODUCTION Nitrate ion is derived from protein etc. as a result of the decomposition by germs and bacteria and therefore, it is considered as an effective index to show human-induced contamination. The water quality standard value for nitrate nitrogen is 10 mg/L or less. In general, about 0.1-1 mg/L of nitrate ion is contained in fresh water. This time, nitrate ion in mineral water was assayed by using the water quality analysis reagent kit for nitric acid (nitrate nitrogen) of Kyoritu KEY WORDS Environmental Analysis Related, Environmental Water, Clean Water,Environmental Chemistry, Environment, Mineral Water, Color Reagent,Calibration Curve, Nitrate lon, NOg, Nitric Acid, Calibration Curve,UV,U-5100,U-1900,U-2900, U-2910, U-3010, U-3310, U-1800, U-2800 Spectrophotometer (UV) Recoveryrate 0.354±0.000 Sheet No. UV100006-01 96.4±0.0% Nitrate ion is derived from protein etc. as a result of the decomposition by germs and bacteria and therefore, it isconsidered as an effective index to show human-induced contamination. The water quality standard value for nitratenitrogen is 10 mg/L or less. In general, about 0.1-1 mg/L of nitrate ion is contained in fresh water. This time, nitrate ionin mineral water was assayed by using the water quality analysis reagent kit for nitric acid (nitrate nitrogen) of KyorituChemical-check Lab. This reagent kit allows the analysis of nitric acid in fresh water in the absence of nitrous acid. U-5100, when used with the reagent kit for nitric acid, allows easy analysis of nitric acid. Because of the automatic 6-cell turret, which is included in the standard installation, the sample switching can also be performed smoothly. METHOD PREPARATION Analyte : NO: Measurement: Reduction+naphthylethylenediamine method method (Simple analysis by using the water quality analysis reagent of Kyoritsu Chemical-check Lab) Reagent : Kyoritsu Chemical-check lab, Water Quality AnalysisReagent Kit No.19 NO3 Assay range : 0.2-5.0 mg/L Refer to JIS K0102 43 and Standard Methods for the Examination of Waterand Wastewater VI-2. 12 for the official method. Water sample 25mL -Add of R-1 reagent (*1)Stir - Add R-2 reagent (*2)Stir For 1 min (about 120 times)· Let it stand for 15 min Solution for measurement Temperature : room temperature *1,*2 Kyoritsu Chemical-check Lab,Water Quality Analysis RagentKit No.19 NO. * For details such as the effects of the interfering substances and reagent, pleasecontact Kyoritsu Chemical-check Lab. INSTRUMENTCONDITIONS Instrument : U-5100 Measurement wavelength1: 539nmScan speed : 400 nm/min Slit : 5 nm
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