饲料添加剂和饲料原料中胆碱（维生素B4）检测方案(毛细管电泳仪)

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Analytical EquipmentHIGH PERFORMANCECAPILLARY ELECTROPHORESISSYSTEMwww.lumexinstruments.ccm DETERMINATION OF CHOLINE (VITAMIN Ba) IN FEEDSTUFFS， COMPOUND FEEDS， FODDERADDITIVES， AND FEED RAW MATERIALS Lumex Method M 04-82-2014 INTRODUCTION The method is used for the determination of the mass fraction of free choline (synthetic and natural) infeedstuffs by capillary electrophoresis. The method can be applied for all types of feedstuffs， compoundfeeds， fodders， premixes， fodder additives， and feed raw materials. The present method does not allowthe determination of bound choline. MEASUREMENTMETHOD The measurement method is based on extraction of free choline (vitamin B4) from a sample by water and itsdetermination by capillary electrophoresis with indirect UV detection at the wavelength of 267 nm. MEASUREMENT RANGE The measurement range for choline (expressed as choline chloride) is 0.01-100%. EQUIPMENT AND REAGENTS The CAPEL capillary electrophoresis system is used in measurements. Data acquisition， collection，processing and output are performed using a personal computer running under WINDOWS XP/7/8/10operating system with installed dedicated software package ELFORUN. Lumex Instruments set， order No 0300001903. EXAMPLES OF REAL ANALYSES BGE： benzimidazole， with tartaric acid Capillary： Leff/ Ltot 50/60 cm， ID 75 m Injection： 150 mbar x sec Voltage： +20kV Temperature： +40°c The contents on this paper are subject to change without notice. To get more specific information， please contact the representative by sales@lumexinstruments.com ' National Standard GOST R57124-2016. 氯化胆碱是B族维生素之一，它可以提高畜禽对氨基酸的利用率，同时具有抗脂肪肝和增强动物体质的作用，因此各类饲料中均添加氯化胆碱，市场需求量很大。一些氯化胆碱生产厂为了获取高额利润，向产品中大量掺假，以降低成本低价出售，因此造成了市场上掺假产品泛滥，使许多消费者蒙受了损失，因此饲料及饲料添加剂中氯化胆碱的测定就变得尤为重要。毛细管电泳法可以测定饲料中游离胆碱（合成的和天然的）的质量分数，但是不适用于测定结合的胆碱。该方法可应用于各种饲料、复合饲料、饲料、预混料、饲料添加剂、饲料原料等。该方法的测定波长为267nm。