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Improving Peak Shape and Recovery in LC Studies of Phosphated Vitamin B2

检测样品预防类生物药品

检测项目含量测定

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安捷伦科技(中国)有限公司

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方案详情文

Accurate analysis of riboflavin diphosphates and monophosphates with the Agilent 1290 Infinity II Bio LC System

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Phosphated vitamin B2 (riboflavin) vitamers were analyzed by UHPLC and a performance comparison was made in terms of peak shape and recovery obtained on an Agilent 1290 Infinity II LC System (classic stainless steel LC) versus an Agilent 1290 Infinity II Bio LC System (biocompatible LC), and on classic versus metal-free column hardware. The results clearly demonstrate the necessity of biocompatible flow paths for accurate analysis of riboflavin diphosphates and monophosphates to prevent nonspecific interactions of these compoundswith the metal surfaces. The iron-free flow path allows the use of volatile MS/ELSD-compatible mobile phases for the direct analysis of the phosphorylated species without the need for enzymatic treatment with phosphatase.

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检测仪器
Agilent 1290 Infinity II 生物液相色谱系统安捷伦液相色谱(LC)
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安捷伦科技(中国)有限公司为您提供《Improving Peak Shape and Recovery in LC Studies of Phosphated Vitamin B2》，该方案主要用于预防类生物药品中含量测定检测，参考标准《暂无》，《Improving Peak Shape and Recovery in LC Studies of Phosphated Vitamin B2》用到的仪器有Agilent 1290 Infinity II 生物液相色谱系统。

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